面向生物膜双向调控的群体感应/群体淬灭对二氯甲烷废气净化的作用机制

以二氯甲烷（DCM）为代表的含氯VOCs废气处理是当前大气污染控制领域的难题。利用生物滴滤（BTF）净化含氯VOCs废气具有较高的应用前景，但是存在BTF启动阶段挂膜难、去除负荷低以及长期运行后床层易堵塞的问题，从而限制了该技术的发展与应用。.本项目拟以DCM为模型污染物，通过群体感应（QS）与群体淬灭（QQ）方法调控BTF在净化DCM废气启动期及稳定期后期的生物膜。分析DCM降解菌成膜关键信号分子在BTF中的时空分布规律，揭示QS-QQ平衡及其偏移机制，剖析QS/QQ调控引发的细胞表面疏水性与生物膜厚度变化而导致的DCM传质改变规律，解析调控过程中BTF净化废气的传质-反应动力学，阐明QS/QQ对DCM废气生物净化的作用机制，获得分别面向BTF启动期与稳定期后期生物膜的科学调控策略，从而实现废气中DCM的高效、稳定去除，为QS/QQ技术在含氯VOCs废气生物净化中的工程应用奠定基础。

The treatment of the waste gas containing chlorinated volatile organic compounds (VOCs), e.g. dichloromethane (DCM), is a difficult issue in the field of air pollution control. The biotrickling filter (BTF) has exhibited a promising application prospect in the purification of this kind of waste gas recently. However, it is still suffering from the great difficulty of biofilm formation and the low elimination capacity at start-up period and the frequent occurrence of bed clogging during long-term operation, limiting the further development and application of BTF..In this study, DCM is employed as the model pollutant. Quorum sensing (QS) and quorum quenching (QQ) will be utilized to regulate the formation of biofilm in the BTF at start-up and late steady periods, respectively. The temporal and spatial distribution of the key signal molecule for biofilm formation in DCM removal will be analyzed. The mechanisms of QQ-QS balance maintenance and deviation during BTF operation will be revealed. The changes of cell surface hydrophobicity and biofilm thickness due to QS/QQ regulation and the resulting variations of DCM mass transfer will be explored. Then, the dynamic model for the mass transfer and reaction of DCM in BTF under the regulation will be investigated. Based on these results, the mechanism of QS/QQ influencing the biological treatment of DCM-containing wastegas will be clarified. The reasonable strategy for controlling the biofilm in BTF at start-up and late steady periods, respectively, will be subsequently obtained. Thus, the steady purification of DCM with high efficiency would be realized, which lays sound foundation for the application of QS/QQ technology in the enhancement of the treatment of waste gas containing chlorinated VOCs.