刺激响应型光电化学传感系统及抗生素抗性基因的检测研究

抗生素抗性基因(ARGs)的环境污染日益严峻，是亟待解决的重大环境难题。发展快速、灵敏、准确高通量检测ARGs的方法迫在眉睫。本项目拟研究高灵敏多重光电化学检测ARGs新策略，引入刺激响应型智能单元来功能化DNA组装体，通过单一或多元化引发机制（温控、酸控、电位调控等）调控组装体的开关行为与信号因子的多重输出。所释放的信号因子作为触发源引发光电化学传感链式反应，增强光电化学响应信号，从而实现高灵敏高通量检测ARGs。系统探究刺激响应型智能单元与光电化学传感性能的内在联系，分析智能单元的开关特性，揭示信号因子与光电活性单元的协同增效机制。结合微流控技术，集成反应腔、检测腔与控制单元，研制集成一体化的刺激响应型光电化学多重检测器，实现环境介质中ARGs的高灵敏多重检测和丰度监测。该项目实施为深入研究环境介质中ARGs污染水平，揭示其在环境中的扩散和传播规律具有重要的科学意义和应用价值。

The overwhelming prevalence of the resistant bacteria and the aggravation of antibiotic resistance genes (ARGs) contamination have been listed as one of the most critical challenges, causing a severe global health crisis. This project aims to construct the stimuli-responsive photoelectrochemical sensing strategies for realizing the highly sensitive and multiplex detection of ARGs in the environmental medium. The stimuli-responsive intelligent unit is smartly utilized as DNA tags. By signal or multiple stimulation approaches (including pH/temperature/potential control), the switchable behavior of the corresponding stimuli-responsive assembly can be regulated, with the multi-output of signal factors. The released signal factors can trigger the photoelectrochemical signal amplification, thereby finally realizing highly sensitive multiplex detection of ARGs. Great efforts are to be devoted to explore the intrinsic relationship systematically between stimuli-responsive “intelligent unit” and "sensing performances", analyze the switchable behavior of the intelligent unit, and disclose the synergistic mechanism between signal factor and photoelectric active unit. Moreover, to meet the ever-increasing demands for more sensitive, high throughput, convenient, and miniature, the microfluidic integrated all-in-one photoelectrochemical multiple detector would be developed to monitor the abundance of ARGs in the environmental medium. The theoretical calculations would be performed to classify the ARGs species. This project would prompt the researches on exploring the pollution level, the diffusion and propagation rules of ARGs in the environmental medium, providing significant scientific and application basis.