脂源性生长因子缓释胶原片段介导的异种脂肪脱细胞基质体内脂肪再生的机制研究

脂肪脱细胞基质（AAM）的商业化应用带来了软组织缺损修复的革命。然而目前AAM产品移植后的组织构建物以纤维结缔组织为主。AAM制备过程中被损耗的脂源性生长因子是影响其体内成脂的主因。在我们前期的研究中，我们用物理法方法成功地提取了无细胞的脂源性生长因子缓释胶原片段（ACF），联合ACF的AAM的移植在体内可成功诱导出高度成熟的脂肪组织。我们通过胶原示踪技术成功追踪到脂肪胶原片段的降解过程，但胶原降解后释放脂源性生长因子的动态过程尚不清楚。据此,我们推测：ACF作为脂肪细胞外基质的胶原片段，能稳定地缓释基质内贮存的脂源性生长因子，诱导血管新生及体内成脂分化，诱发出结构成熟的脂肪组织再生。本研究拟采用胶原示踪标记、质谱分析、流式细胞检测等方法，重点研究调控ACF诱导AAM体内脂肪再生诱导过程中的细胞学与分子生物学基础及相关机制，为实现ACF联合AAM异种体内脂肪再生诱导提供实验和理论依据。

The commercial application of acellular adipose matrix (AAM) has brought a revolution in the repair of soft tissue defects. However, at present, in vivo transplantation of AAM generally lead to the fibrous connective tissue. The loss of adipose- derived growth factors during the preparation of AAM is the main factor affecting its adipogenesis in vivo. In our previous study, we successfully extracted adipose-derived growth factor sustained-release collagen fragment (ACF) by physical method, and the transplantation of AAM combined with ACF can successfully induce highly mature adipose tissue in vivo. We have successfully traced the in vivo degradation process of ACF by collagen tracer, but the dynamic release process of adipose-derived growth factors after collagen degradation is still unclear. Therefore, we speculate that ACF, as adipose extracellualr collagen fragments, can stably release adipose-derived growth factors stored in the matrix, induce angiogenesis and adipogenic differentiation in vivo, leading to the regeneration of mature adipose tissue. In this study, collagen tracer, protein analysis and flow cytometry were used to study the cellular and molecular biological basis of ACF-mediated adipogenesis in AAM, providing experimental and theoretical basis for AAM-based xenogeneic adipose regeneration induction.