保幼激素与蜕皮激素通过USP甲基化/乙酰化调控飞蝗周期性卵黄发生的分子机制

周期性产卵的昆虫通常有多个产卵周期，但对其分子调控机制尚不了解。本项目前期研究发现，保幼激素（JH）与蜕皮激素（20E）分别诱导超气门蛋白（USP）的甲基化修饰与乙酰化修饰，调节卵黄原蛋白（Vg）的周期性表达和产卵周期的形成，但其中的调控机制有待阐明。本项目以飞蝗为模型，鉴定JH和20E分别诱导USP甲基化修饰和乙酰化修饰的信号途径，阐释USP甲基化和乙酰化动态修饰过程中的JH与20E信号通路交互作用，解析USP甲基化和乙酰化动态调控Vg表达和周期性卵黄发生的分子机制。研究结果对揭示昆虫生殖过程中的JH和20E互作、昆虫周期性产卵以及强大产卵能力的分子机理具有重要意义。

In general, the periodic oviposition insects have several oviposition cycles to enhance the fertility, but the molecular mechanisms remain elusive. Previous studies in this project found that juvenile hormone (JH) and 20-Hydroxyecdysone (20E) triggered the posttranslational modification of ultraspiracle protein (USP) by lysine methylation and lysine acetylation, respectively to mediate the periodic expression of vitellogenin (Vg) and the formation of oviposition cycles. But again, the underlying mechanisms are poorly understood. Using the migratory locust Locusta migratoria as a model system, we aim to explore the signaling transduction pathways involved in the regulation of JH and 20E in the USP methylation and acetylation, respectively, unveil the interaction between JH and 20E signaling pathways, and elucidate the regulation of periodic Vg expression and vitellogenesis by USP methylation and acetylation. This study will be significant to reveal the interplay between JH and 20E during the process of adult reproduction, and the molecular mechanism of the periodic oviposition and strong fecundity in insects.