草鱼出血病发生的分子机制解析

草鱼呼肠孤病毒（GCRV）引起的草鱼出血病给水产养殖业造成了巨大的经济损失。草鱼出血病的发生一方面由于草鱼免疫系统稳态平衡被GCRV打破，另一方面GCRV经过长期进化也获得了逃避草鱼免疫防御系统的机制。因此，基于前期在个体和细胞水平上对II型GCRV抗性/易感及病毒反向遗传操作系统的初步研究，本项目拟从宿主免疫应答失衡与病毒免疫逃逸两个方面，重点开展II型GCRV引起的草鱼先天性与获得性免疫应答失衡以及II型GCRV针对草鱼干扰素与抗原递呈等系统的免疫逃逸机制研究，同时通过反向遗传操作系统构建新型GCRV减毒疫苗。本项目的开展将系统解析草鱼出血病的发生机制并探索其免疫预防策略，具有重要的理论意义和实践价值。

Grass carp reovirus (GCRV) causes grass carp hemorrhagic disease and great economic losses in aquaculture. The occurrence of grass carp hemorrhage is at one aspect due to the striking of grass carp immune system homeostasis caused by GCRV. Meanwhile, GCRV also acquires ability to evade the grass carp immune system during the evolution. Therefore, based on our previous research on the resistance/ susceptibility and reverse genetics of group II GCRV at individual and cell levels, this proposal will conduct experiments from two aspects including the host immune responses and viral immune evasion, with the focus on innate and acquired immune imbalance of grass carp caused by group II GCRV and immune evasion mechanisms of group II GCRV against host interferon and antigen presentation systems. Meanwhile, the reverse genetics system will be used to generate GCRV attenuated vaccine. The accomplishment of this proposal will illuminate the occurrence mechanism of grass carp hemorrhage and explore the preventative strategy, and have important theoretical and practical meanings.