基于“肠道菌群-SCFAs-SIgA”途径探讨葛根芩连汤治疗AAD的机制

肠道菌群是人体健康状况的晴雨表。研究表明肠道菌群影响其短链脂肪酸代谢, 短链脂肪酸能促进B细胞分化为浆细胞，分泌免疫球蛋白A（IgA）,与肠上皮细胞分泌片组成SIgA，发挥免疫效应。文献及课题组前期研究发现葛根芩连汤能调节肠道菌群结构及短链脂肪酸代谢，从而改善抗生素相关性腹泻。为此，本课题组提出：葛根芩连汤通过“肠道菌群-短链脂肪酸-SIgA”途径干预抗生素相关性腹泻的科学假说。本项目拟通过葛根芩连汤干预抗生素相关性腹泻大鼠模型，应用16S rRNA序列分析其肠道菌群结构变化；GC-MS检测肠道粪便短链脂肪酸代谢；流式细胞术和RT-PCR等方法分析影响肠道B细胞转化浆细胞相关因子，并对肠道菌群及短链脂肪酸，浆细胞分泌IgA进行相关性分析，揭示葛根芩连汤干预抗生素相关性腹泻的部分机制，为该方临床合理应用提供科学依据。

Gut microbiota is considered to be a barometer of human health.Studies had shown that intestinal flora affected the metabolism of short-chain fatty acids. Short-chain fatty acids culd promote the differentiation of B cells into plasma cells, secrete immunoglobulin A (SIgA), and formed SIgA with intestinal epithelial cells secreted tablets, exerting immune effects. Literature and previous studies of the research group found that gegenqinlian decoction could regulate intestinal flora structure and short chain fatty acid metabolism, so as to improve antibiotics-related diarrhea. Therefore, the applicant of this subject put forward the scientific hypothesis that Gegen Qinlian Tang is based on the "Gut microbiota-SCFAs-SIgA" approach to treat antibiotic-related diarrhea. In order to verify this hypothesis, this project plans to prepare AAD rat models through antibiotics, use GQT to intervene, and use molecular microbial ecology 16S rRNA sequences to analyze the changes in the gut microbiota of experimental animals. GC-MS detection of the metabolism of SCFAs ; Flow cytometry and RT-PCR and other methods to analyze the relevant indicators that affect the secretion of IgA by intestinal plasma cells. Correlation analysis ofg ut microbiota and its metabolic SCFAs, plasma cell secretion of IgA. The results of the study are intended to reveal some of the mechanisms of GQT in the treatment of AAD, and provide a scientific basis for the reasonable clinical application of this side.