激素和营养信号通路协同调控中黑盲蝽生殖的分子机制

激素和营养信号通路在许多昆虫的生殖发育中发挥重要作用，申请人前期研究发现沉默中黑盲蝽雌虫保幼激素受体Met，蜕皮激素受体EcR，胰岛素受体InR，均极显著降低了终生产卵量；中黑盲蝽补充动物营养后，TOR基因表达量极显著上升，终生产卵量极显著升高，但激素和营养信号通路在中黑盲蝽生殖调控中的功能，以及它们之间是否存在交互作用（Crosstalk）值得深入研究。因此，本项目拟采用信号通路阻断剂和RNA干扰等方法，研究激素和营养信号通路及其关键基因对中黑盲蝽生殖发育的调控作用，同时研究激素和营养信号通路中关键基因之间的互作关系，最终解析营养与激素信号通路的协同调控中黑盲蝽生殖的机制。研究结果不仅对揭示杂食性昆虫生殖调控的分子机制具有重要的理论意义，同时为制定新型高效的害虫防治策略提供新的科学依据。

Hormone and nutritional signaling pathways play important role in insect reproductive development. Our previous data proved that all the juvenile hormone, ecdysone, TOR pathway and insulin signaling pathway are involved in reproductive regulation in Adelphocoris suturalis. furthermore, after having animal nutrition, the lifetime oviposition of A. suturalis female significantly increased. But the regulated function of hormone and nutritional signaling pathways, and their cross-talk between them in co-regulation of reproductive development were still unclear. The present project aims to analyze the regulation function of both hormone and nutritional signaling pathways and their cross-talk mechanism in regulation of reproductive development through RNAi, western-blot and qRT-PCR approaches. The expected results will help in illustration of the molecular mechanisms underlined reproductive regulation of omnivorous insect, and also in developing new pest management strategies.