中华鳖甘露糖受体MRC1和MRC2抗细菌感染的功能及分子机制研究

细菌性疾病在中华鳖养殖中频频爆发，危害严重，但目前尚缺乏有效治疗手段。甘露糖受体是医学热点研究的模式识别受体，具有启动固有免疫和适应性免疫的功能，其常被作为靶点受体用于新型药物和疫苗的开发。中华鳖甘露糖受体是否具有哺乳动物同样的免疫抗病功能，并被用于靶向新药开发尚待进一步研究。本项目拟通过体外结合实验和细胞吞噬实验确证MRC1和MRC2是病原结合和吞噬受体，然后鉴定其调节表达的下游细胞因子，阐明MRC1和MRC2的免疫调节功能；最后利用磁珠亲和层析、质谱测序以及免疫沉淀等技术筛选和鉴定MRC1和MRC2的接头蛋白，全面揭示中华鳖甘露糖受体抗感染功能及分子机制。本项目研究成果不但揭示了水产动物MRC1和MRC2与高等哺乳动物同源受体的关系，而且对深入了解中华鳖甘露糖受体抗感染免疫机制，以及开发鳖类甘露糖受体靶向新药和疫苗都具有重要意义。

Bacterial diseases have frequent outbreak in the Pelodiscus sinensis industry, which is seriously harmful, but there is still no effective prevention and treatment. Mannose receptors as recognition receptors，are medical research hotspot model, which have functions that initiate inherent immunity and adaptive immunization, and are often used as a target receptor for new drugs and vaccines. Whether the mannose receptors of P. sinensis has the same immune functions as mammals, and could be target gene to develop new drugs and vaccines still to be further studied. This project is proposed to confirm that MRC1 and MRC2 are phagocytic receptors of pathogens by in vitro binding experiments and cell phagocytosis experiments, and then identify the downstream cytokines of these two mannose receptors; finally the downstream linking proteins of MRC1 and MRC2 will be identified. The results of this project not only reveals the relationship between the aquatic animal MRC1 and MRC2 and the higher mammalian homologous receptor, but also understand the immune mechanism of the P. sinensis mannose receptors. More, the results of this project will be of great significance for the development of new drugs and vaccines that targeted to mannose receptors.