基于味蕾类器官和多模态集成芯片的仿生传感器及其响应机理研究

仿生味觉传感器在食品安全、生物医学、环境保护等领域具有广阔的应用前景和开发价值。本项目针对当前仿生味觉传感器研究中亟待解决的难题，开展基于味蕾类器官和多模态集成芯片的仿生传感器及其响应机理研究。首先，针对如何获得功能性敏感材料这一难题，基于味觉干细胞和悬滴网络微流控芯片，制备功能性味蕾类器官，并结合传感器监测数据进行质量控制；其次，针对如何高通量、多参数、实时检测味蕾类器官的响应这一难题，基于阻抗传感器、微电极阵列和光寻址电位传感器开发多模态集成传感芯片，检测和采集味蕾类器官的阻抗、膜电位、胞外离子变化和神经递质释放等味觉响应信息，建立味觉响应模型，揭示味觉信号响应机理，以提高传感器对味觉物质的检测性能。本项目创新性地结合类器官和多模态集成传感技术开发新型仿生味觉传感器，为味觉物质检测和味觉传感机理的研究提供新的技术手段，有望在功能性类器官制备和仿生味觉传感技术方面取得一定进展和突破。

Biomimetic taste biosensors have shown promising prospects and broad applications in many fields such as food safety, biomedicine, and environmental protection. This project aims at the urgent problems that need to be solved in the current research of biomimetic taste biosensors. This project focuses on the research of the biomimetic taste biosensor and the responsive mechanisms based on taste bud organoids and multimodal-integrated chips. Firstly, to solve the problem of how to obtain functional sensitive materials, functional taste bud organoids are prepared based on taste stem cells and hanging-drop network (HDN) microfluidic chips. The quality of functional taste bud organoids is monitored and controlled via the feedback data of biosensors. Secondly, to solve the problem of how to detect the responses of taste bud organoids in a high-throughput, multi-parameter, and real-time manner, multimodal-integrated sensing chips are developed by integrating impedance sensor, microelectrode array (MEA), and light-addressable potentiometric sensor (LAPS), which can detect and collect taste responsive information from taste bud organoids including impedance, membrane potential, extracellular ion concentrations, and neurotransmitter release. Taste responsive model is built to reveal taste signal responsive mechanisms, which aims to improve the performances of biomimetic taste biosensors for the detection of taste substances. This project innovatively combines organoids and multimodal-integrated sensing technology to develop novel biomimetic taste biosensors, which also provides a new platform and technical approach for the detection of taste substances and research of taste signal transduction mechanisms. This project is expected to achieve certain progress and breakthroughs in preparation of functional organoids and biomimetic taste sensing technology.