拟南芥nlp7突变体抑制子的筛选、鉴定及作用机制研究

转录因子NLP7（NIN-like protein 7）在硝态氮信号调控网络中处于主导地位，深入解析NLP7介导的硝态氮调控网络对提高氮素利用率具有重要的研究意义。本项目在利用正向遗传学方法筛选获得nlp7突变体抑制子的基础上，克隆出了新的NLP7调控网络的调节子NSU。拟进一步从形态、生理生化、分子水平解析NSU基因的功能，阐明NSU基因调控硝态氮转运、同化及信号的机理；利用转录组学研究NSU基因在硝态氮代谢及信号调控方面的作用机制和信号通路；利用生物化学和遗传分析明确NSU与NLP7之间的调控关系及作用机制，充实和完善NLP7调控网络；挖掘NSU基因与其他硝态氮调控基因之间的关系，丰富硝态氮信号调控网络。预期结果为破译NLP7介导的硝态氮信号调控网络奠定基础，为培育氮高效作物新品种、提升农业可持续发展提供新的线索和理论依据。

The transcription factor NLP7 plays a dominant role in the nitrate signaling and regulatory network. Identification and characterization of more nitrate regulatory genes in the NLP7-mediated nitrate regulatory network are of great importance for improving nitrogen use efficiency. In this research, we acquired a suppressor of nlp7 by a genetics screen and cloned a new nitrate regulatory gene NSU. We will investigate the function of NSU from morphological, physiological, biochemical and molecular levels. Transcriptomics will be used to test the signaling pathway of NSU gene in nitrate metabolism and signal regulation. We will further test the regulatory relationship and mechanism between NSU and NLP7 by biochemical and genetic analyses and enrich the regulatory network of NLP7. Furthermore, we will also investigate the relationship between NSU and other nitrate regulatory genes. The expected results will lay a foundation for decoding the NLP7-mediated nitrate signaling and regulatory network, and provide new clues and theoretical basis for breeding new crop varieties with high nitrogen use efficiency and promoting agricultural sustainable development.