贵州苗医药方“四大血”对类风湿性关节炎滑膜血管翳的影响及其作用机制的研究

类风湿性关节炎(Rheumatoid arthritis，RA)是一种慢性、炎症性自身免疫系统疾病，目前无有效治疗手段。其作为血管源性的炎性疾病，重要病理特征是血管翳形成，以此为靶标的疗法成为治疗RA潜在策略。而贵州苗族地区治疗RA的特有药方“四大血”应用历史悠久、疗效独特、毒副作用小，尚处于经验用药阶段。项目组前期动物实验结合网络药理学、生物信息学分析结果提示“四大血”可能通过调控VEGF/PI3K/AKT信号通路改善RA滑膜血管翳，但具体作用机制不清。因此，本项目综合运用多学科知识，研究“四大血”对RA滑膜血管翳的影响；并基于VEGF/PI3K/AKT信号通路，利用RNA干扰、蛋白组联合转录组分析、基因敲除等技术深入研究“四大血”作用RA滑膜血管翳的分子机制，以期为干预和治疗RA提供新靶点并为“四大血”的临床运用提供科学依据。

Rheumatoid arthritis (RA) is a chronic, inflammatory autoimmune disease, for which there is no effective treatment at present. As a vascular inflammatory disease, pannus formation is one of the important lesion characteristics, and the therapy aiming at the synovial pannus becomes a potential strategy for the treatment of RA. Actually, the “Sidaxue” prescription, which has been a specific medicine prescription for RA treatment in Miao nationality area of Guizhou Province for a long history, has unique efficacy and less side effects, but is still in the stage of experience medication. The project results of earlier animal experiments combined with network pharmacology and bioinformatics analysis suggested that “Sidaxue” might improve RA synovial pannus by regulating VEGF / PI3K / AKT signal pathway, but the specific mechanism was unclear. Therefore, we intend to comprehensively adopt multi-disciplinary methods to study the influence of “Sidaxue” on RA synovial pannus. We will use RNA interference, Proteomics combined transcriptome analysis, Gene knockout and other technologies to further study the mechanism of “Sidaxue” on RA synovial pannus based on VEGF / PI3K / AKT signal pathway in order that we can reveal new targets for intervention and treatment of RA and provide scientific basis for clinical application of “Sidaxue”.