南疆策勒黑羊多胎性状QTLs的遗传解析

策勒黑羊主产于新疆南疆和田地区策勒县，以多胎性状著称。多胎性状属阈性状，不同绵羊品种的多胎性状主效基因不同，已报道该性状QTLs有67个。申请者建立在策勒黑羊和和田羊高密度基因组芯片扫描分析的基础上，经选择信号分析发现51个分子标记和策勒黑羊繁殖性状相关。可见策勒黑羊繁殖性状并不是由一个或几个主效基因控制的绵羊，反而有更多的分子标记控制。. 因此本项目选择策勒黑羊的多胎性状为研究对象，建立不同产羔数的资源群体，对其制备绵羊基因组高密度芯片，进行全基因组关联分析；分别对单羔性状和多羔性状的资源群体进行选择信号分析；为将来通过分子手段选育适应本地气候的南疆绵羊多胎品系或品种提供新的思路。

Cele Black Sheep is known as litter size in Cele county, wihch is produced in Hetian district South Xinjiang. Litter size is threshold trait, and major genes of litter size is different in different sheep breeds. Currently, 67 QTLs have been reported.Based on the scanning analysis of high-density genomic microarray of Cele Black Sheep and Hetian Sheep, applicants found that 51 molecular markers were associated with reproductive traits of Cele Black Sheep by selective signal analysis. It can be seen that reproductive traits of Cele black sheep are not controlled by one or several major genes, but controlled by more molecular markers.. Therefore, this project selects litter size of Cele Black Sheep as research object, establishing resource groups with different litter sizes. Then the project makes genome-wide association analysis with high-density genomic microarray. Intra-group and inter-group selective signal analysis of resource groups is carried out, and these resources groups includes single lamb trait groups and multi-lamb trait groups.This study attempts to analyze genetic basis of fecundity traits of Cele Black Sheep, which will provide new ways for breeding sheep with fecundity traits that adapt to local climate of South Xinjiang through molecular method.