基于LA-HR-ICP-MS/ORS-ICP-MS生物体硒、汞元素含量与分布同步测试方法的建立及应用

随着原位微区分析技术的发展，不仅可以对生物组织进行定量、半定量成像，还可以对单根发丝进行更详细的分段分析，反演身体元素的历史水平。本项目拟建立一套基于LA-HR-ICP-MS/ORS-ICP-MS同步测定Se和Hg元素含量及分布的方法，利用该方法测定万山汞矿区不同汞污染环境中居民头样中的硒、汞含量，研究时间序列上人体汞暴露水平及硒与汞的作用关系。另外，以大鼠为研究对象进行硒、汞毒性实验，利用LA-HR-ICP-MS/ORS-ICP-MS测定其器官组织中硒汞的含量并同时进行成像分析，探讨硒对汞生物学行为的影响。这种原位微区分析技术不仅可以为新型汞暴露健康风险评价提供可靠的技术支持，还能更直观地分析硒对生物体内汞的生物学行为及毒性效应的影响，是研究万山汞矿区居民汞暴露健康风险评价重要内容。

In-situ analysis technique plays a key role depending on its less pollution, high efficiency and high spatial resolution. It was used to produce images of detailed regionally-special element distribution in thin sections of different parts of biological samples, besides, the spatial resolution for a single human hair investigated by LA-ICP-MS could translate into a high time resolution of trace element levels over short intervals. In our study, analysis method of selenium (Se) and mercury (Hg) achieved by LA-HR-ICP-MS/ORS-ICP-MS will be established for biological samples, including human hair and rat tissues. Based on this method, the contents of Hg and Se in human hair samples from people living in Wanshan Hg mining area are determined to figure out the relationship of Hg and Se and health risk of Hg exposure. Additionally, Hg/Se-laden rat tissues are obtained through toxicity test of MeHg and SeMet. Contents and bioimaging of Hg and Se in rat tissue by LA-HR-ICP-MS/ORS-ICP-MS will be conducive synchronously to analyze the influence of Se to Hg uptake and translocation. In conclusion, the in-situ LA-HR-ICP-MS/ORS-ICP-MS analyses of Se and Hg in biological samples could supply a reliable technique for new-type health risk assessment, and it could reveal the inhibit of Se to the biological behavior of mercury intuitively. Moreover, our work is substantial part for human health risk assessment living in Wanshan Hg mining area, even for researching environmental Hg pollution.