花烛苞片类胡萝卜素生物合成途径的特征及其调控机制研究

花烛为天南星科花卉，因其苞片颜色鲜艳而深受大众喜爱。尽管其栽培品种已较为丰富，但目前黄色苞片品种仍较少，培育苞片呈黄色的优良花烛新品种可以进一步丰富其品种类型，促进我国花烛产业的发展。由于植物组织中黄色色素积累主要受类胡萝卜素代谢途径的影响，为探明花烛苞片类胡萝卜素积累的特征，本研究拟在已完成不同颜色苞片花烛代谢组分析的基础上，进一步对不同颜色苞片进行转录组测序和分析，通过综合分析明确不同颜色苞片类胡萝卜素代谢途径的基因表达特征，并鉴定调控转录因子，探明花烛苞片类胡萝卜素积累较少的原因。在此基础上进一步克隆关键基因和转录因子，构建过表达及干扰表达载体，通过瞬时转化及稳定转化花烛，筛选类胡萝卜素含量显著提高的花烛株系，温室移栽至开花后筛选类胡萝卜素含量显著提高苞片呈深黄色的优良株系，研究结果将为花烛及其他天南星科花卉苞片颜色的改良提供参考。

Anthurium andraeanum Lind. belongs to the Araceae family. Because of its colorful spathe and bright leaves, anthurium has been a very popular flower in China and worldwide. However, there are only a few yellow color cultivars at present. That breeding new anthurium cultivars with yellow spathe will improve the cultivation of anthurium in China. The yellow pigments in flower organs are carotenoids. However, there is only a small amount of carotenoids accumulation in anthurium spathe. It is difficult to display the yellow color in spathe. So far, the characteristic of carotenoid biosynthesis in anthurium remains unclear. In this study, we will investigate the gene expression characteristics of carotenoids biosynthetic pathway in anthurium spathe and to elucidate the reason of the lack of carotenoids in anthurium. The experiments include transcriptome analysis of carotenoids in different anthurium cultivars, analysis of expression levels of genes and transcription factors, chromoplast morphology, cloning of key genes and transcription factors, construction of overexpression and RNA interference vectors, transient expression and stable genetic transformation. This study will promote the understanding of carotenoids biosynthesis in anthurium and creating of new germplasms for anthurium and other aroids.