烟粉虱MED隐种响应温度胁迫的染色质重塑调控机制

烟粉虱MED隐种是一种世界性分布的重要入侵害虫。前期研究表明，该物种具有较强的快速适应极端环境温度的能力，表观遗传修饰是其快速应对逆境温度的一个重要途径。本项目以表观遗传因子染色质重塑复合物对胁迫基因的表达调控为切入点，围绕烟粉虱“响应温度胁迫的染色质重塑调控机制”这一科学问题，采用转录组测序、ATAC测序以及单碱基编辑等技术，从转录调控网络和碱基功能位点变异两个维度来探究染色质重塑复合物对烟粉虱温度胁迫下的调控机制。主要内容包括：1）首先筛选响应极端温度胁迫的关键染色质重塑复合物；2）然后解析关键染色质重塑因子对染色质结构的调控；3）进一步构建染色质重塑与胁迫基因之间的表达调控网络；4）最后阐明关键染色质重塑因子功能突变点对烟粉虱温度胁迫适应的调控机制。项目最终揭示烟粉虱对温度胁迫响应的分子遗传调控机制，为全球气候变化下广布性昆虫的温度适应调节和扩张机制提供较为系统和全面的理解。

Bemisia tabaci Mediterranean (MED) cryptic species is an important invasive pest which has a worldwide distribution. The previous studies indicated that the species has a strong ability to adapt to extreme environmental temperature quickly, and epigenetic modification is an important way to deal with the adverse temperature quickly. In this project, we select the expression of stress genes regulated by the epigenetic factor chromatin remodeling complex as the starting point and focus on "the regulatory mechanism of chromatin remodeling in response to temperature stress". And adopting transcriptome sequencing, ATAC sequencing and single base editing to explore the epigenetics regulatory mechanism of B. tabaci under temperature stress through transcriptional regulatory network and base function site variation. The main contents include: 1) screening the key chromatin remodeling complex in response to extreme temperature stress; 2) analyzing the regulation of key chromatin remodeling factors on chromatin structure; 3) further constructing the expression regulatory network between chromatin remodeling and stress genes; 4) finally clarifying the regulation mechanism of key chromatin remodeling factors’ functional mutation sites to temperature stress. These results would provide support to understand the molecular genetic regulation mechanisms of temperature adaptation in B. tabaci, and expansion of widely distributed insects in the context of global change.