基于外源ABA作用的红砂抗旱生理及分子响应机制研究

随着全球气温的不断升高，干旱、半干旱区植物的生境将越来越严酷，大多数植物将面临.干旱胁迫。干旱胁迫下植物通过根系合成和输送ABA来调节自身的生理机能以达到抵御干旱的目的。外源ABA处理也能提高植物的抗旱能力，但外源ABA处理提高植物抗旱能力的生理和分子机理尚不明确，有待揭示。本项目以我国干旱荒漠区典型的超旱生灌木红砂为研究对象，采用自然干旱法，探讨在持续干旱胁迫下，红砂叶片ABA合成与土壤含水量之间的关系，确定干旱胁迫影响红砂合成ABA的土壤含水量阈值；进而采用根源信号物质ABA处理红砂苗木，揭示外源物质作用下红砂抗旱的生理和分子响应机制；为提高半干旱干旱区植物的抗旱能力提供参考价值，也为荒漠区红砂种质资源保护提供方法和理论支撑。

With the global temperature rising, the plant habitat in arid and semi-arid area will be more and more severe because of high temperature and drought stress. Plants regulate their physiological functions through synthesis and transportation of ABA under drought stress in order to resist drought. Exogenous ABA treatment could improve the drought resistance of plants, but the physiological and molecular mechanisms of exogenous ABA treatment to improve the drought resistance of plants have not study yet. This project will take Reaumuria soongorica which is super drought shrub in arid and semi-arid area in China as research project using natural drought method, in order to investigate the relationship between ABA content in R. soongorica leaves and soil water content during continuous drought stress, and ascertain the critical threshold value and threshold limit value of soil water content which affect the ABA content synthesis in R. soongorica leaves, reveal physiological and molecular response mechanism of R. soongorica seedlings spraying different concentration of exogenous ABA when the critical threshold value and threshold limit value of soil moisture content appear. The results obtained from this project will provide reference value for improving drought resistance ability of plants in arid and semi-arid Area, and provides methods and theoretical support for the protection of R. soongorica germplasm resources in desert area.