淫羊藿苷联合诱导多能干细胞分化的肾脏祖细胞治疗急性肾损伤的实验研究

研究证实肾脏损伤后会激活体内的肾脏祖细胞参与损伤修复。多项研究发现淫羊藿苷具有保护受损肾脏、促进肾脏损伤修复的作用，但该过程中淫羊藿苷与成体肾脏祖细胞的关系未见文献报道。研究表明成体肾脏存在Wnt应答肾脏祖细胞，而淫羊藿苷具有激活Wnt信号通路的作用，因此申请人推测淫羊藿苷在参与肾脏损伤修复过程中可能促进成体肾脏祖细胞增殖。已有研究表明外源（多能干细胞分化产生的）肾脏祖细胞具有促进肾脏损伤修复的作用，但治疗效果并不理想。前期研究发现，多能干细胞诱导产生的肾脏祖细胞存在非常高的异质性，本研究拟根据分子标志物将外源肾脏祖细胞分群，利用疾病模型筛选出治疗急性肾损伤最佳的亚群。总之，本研究拟阐明淫羊藿苷与急性肾损伤修复过程中的成体肾脏祖细胞的关系，同时筛选出治疗急性肾损伤效果最佳的外源肾脏祖细胞亚群，最后探讨淫羊藿苷联合外源肾脏祖细胞（亚群）用于治疗急性肾损伤的作用及机制，为临床治疗提供实验支持。

It has been confirmed that kidney progenitor cells can be activated to participate in the repair of renal injury. Many studies have found that icariin can protect the damaged kidney and promote the repair of kidney damage, but the relationship between icariin and adult kidney progenitor cells has not been reported. The results show that there are Wnt responsive kidney progenitor cells in the adult kidney, and icariin can activate Wnt signaling pathway, so the applicant speculates that icariin may promote the proliferation of the adult kidney progenitor cells in the process of renal injury and repair. Previous studies have shown that exogenous (derived from pluripotent stem cells ) kidney progenitor cells can promote the repair of renal injury, and the therapeutic effect is not ideal. Previous studies have found that the kidney progenitor cells induced by pluripotent stem cells have very high heterogeneity. In this study, according to the molecular markers, the exogenous kidney progenitor cells are grouped, and the disease model is used to select the best subgroup for the treatment of acute renal injury. In conclusion, this study aims to elucidate the relationship between icariin and adult kidney progenitor cells in the process of acute renal injury repair, and to select the best exogenous kidney progenitor cell subsets for the treatment of acute renal injury. Finally, to explore the role and mechanism of icariin combined with exogenous kidney progenitor cells (subsets) in the treatment of acute renal injury, so as to provide experimental basis for clinical treatment.