基于多组学整合分析的枸杞多糖缓解全氟辛酸致仔鼠生殖损伤机制研究

全氟辛酸（PFOA）是一种新型、全球性、持续性有机污染物，具有生物蓄积性、难降解性和生物毒性等特点，严重影响人类和动物的生殖健康，已成为环境科学和生物医学领域的研究热点问题。申请者前期研究证实，枸杞多糖（LBP）能够显著缓解PFOA诱导的仔鼠生殖损伤，但其作用机制尚不清晰。在前期研究基础上，本项目深入开展LBP保护生殖健康的调控途径及机制研究。利用转录组学-蛋白质组学-代谢组学整合分析、TUNEL和RT-PCR等方法，重点研究：(1)筛选出与LBP生殖保护作用相关的基因、蛋白质和代谢产物；(2)构建差异基因、蛋白、代谢物，代谢物相关酶及代谢通路的互作网络图；(3)探讨LBP对细胞凋亡的阻断作用及相关基因的表达；(4)明确LBP对下丘脑-垂体-性腺轴及生殖激素的调节作用。研究结果将丰富PFOA生殖损伤拮抗药物资料，为保护动物生殖健康提供理论依据，对促进中药的开发与应用具有重要意义。

Perfluorooctanoic acid (PFOA) is a novel global persistent organic pollutant with a long-term residual effect, bioaccumulation properties, and high toxicity. It severely impacts the reproductive health of humans and animals and has recently been a matter of contention in the field of environment science and biomedicine. Previous studies have demonstrated that Lycium barbarum polysaccharide (LBP) significantly alleviates the PFOA-induced reproductive damage in offspring mice. However, its mechanism has not yet been reported. This study aims to elucidate the regulatory pathways and mechanisms of LBP that protects the animal’s reproductive health. By integrative transcriptomics, proteomics, and metabolomics analysis, TUNEL, and RT-PCR, the following studies will be carried out: (1) The genes, proteins, and metabolites related to the reproductive protection of LBP will be screened out; (2) The interaction network of differential genes, differential proteins, metabolites, metabolite-related enzymes, and metabolic pathways will be determined; (3) The suppressive effect of LBP on cell apoptosis and the expression of related genes will be investigated; (4) Furthermore, the regulatory effect of LBP on the hypothalamic-pituitary-gonadal axis and reproductive hormones will also be clarified. These results will help discover potential drugs that prevent PFOA-induced reproductive damage, provide a theoretical basis for the protection of animal reproductive health, and promote the development and application of traditional Chinese medicine.