StTatC参与马铃薯应答低温胁迫的作用机理

马铃薯是世界第三大重要农作物，低温胁迫使马铃薯生产受到严重危害。因此，开展马铃薯耐受低温胁迫的研究意义重大。前期研究发现：StTatC应答了马铃薯低温胁迫，且基因区甲基化修饰发生了改变，但具体机理未知。本项目拟获得StTatC过表达和CRISPR马铃薯，然后以未转基因马铃薯为对照，比较这些马铃薯的发育表型、对低温的耐性及叶绿体数目、结构的变化，来确定StTatC的生物学功能；利用RNA-seq技术对这些马铃薯进行转录组分析，获得参与StTatC应答低温的相关基因；通过酵母双杂交、BiFC及CoIP技术获得与StTatC互作蛋白，更加全面了解StTatC发挥功能的分子基础；利用BSP、VIGS和PAGE技术揭示DNA甲基化修饰调控StTatC表达的作用。该研究结果可明晰StTatC在马铃薯应答低温胁迫中的作用机理，为后续马铃薯耐低温育种与生产提供理论指导与基因储备，保证我国马铃薯高产稳收。

Potato is the third most important crop worldwide. Low temperature severely influences potato production. Therefore, it is significant to study how to response to low temperature stress in potato. In the early research, we found the gene of StTatC responding to low temperature, and DNA demethylation was found in the gene body of StTatC. However, the molecular mechanism of gene StTatC responding to low temperature is not clear..In this study, StTatC overexpress and CRISPR potatoes will be obtained, and then developmental phenotype, tolerance to low temperature and changes in chloroplast will be analyzed to determine the biological function of StTatC. According to the above data, the further experiments will be designed to determinate the related genes involved in StTatC response to low temperature stress in potato by RNAseq. Yeast Two Hybrid(Y2H), bimolecular fluorescence complementation(BiFC) and co-immunoprecipitation(CoIP) will be used in this research to screen the protein interaction with StTatC, which will benefit more understanding of role of StTatC. BSP, VIGS and PAGE will be used to reveal the regulation of StTatC by DNA methylation..The aim of this research is to explore the mechanism of StTatC in response to low temperature stress in potato, and eventually to benefit the development of potato molecular genetic breeding and production.