中华甲虫蒲螨对草地贪夜蛾的寄生效应及致死机制研究

中华甲虫蒲螨是一种寄生鳞翅目、鞘翅目和同翅目等群居和隐蔽性害虫的外寄生螨，具有繁殖快、毒力强、雌雄比极高和容易饲养等特点。草地贪夜蛾是一种跨境迁飞性重大农业害虫，于2019年1月入侵我国，严重威胁我国粮食作物安全。生物防治是草地贪夜蛾长期有效控制策略之一，而我国缺乏其自然天敌资源，因此很有必要筛选我国本土天敌资源。我们前期研究发现中华甲虫蒲螨能寄生致死草地贪夜蛾所有虫态，蒲螨寄生致死寄主可能通过干扰寄主发育和抑制其免疫反应。本项目拟深入研究中华甲虫蒲螨对草地贪夜蛾的寄生选择性、致死效应以及中华甲虫蒲螨寄生对草地贪夜蛾的免疫抑制作用和对草地贪夜蛾转录组水平的影响等内容。研究结果对明确中华甲虫蒲螨对草地贪夜蛾的寄生效应，获得与寄生致死草地贪夜蛾相关的发育和免疫关键基因，揭示中华甲虫蒲螨寄生草地贪夜蛾的致死机制等方面具有重要的科学意义，同时也为利用中华甲虫蒲螨高效防控草地贪夜蛾提供理论基础。

Pyemotes zhonghuajia Yu, Zhang & He (Prostigmata: Pyemotidae) is an ectoparasitic mite with great potential for use in controlling Lepidoptera, Coleoptera and Homoptera. This ectoparasitic mite has the characteristics of fast reproduction, strong virulence, high female sex ratio and easy breeding. The fall armyworm (FAW), Spodoptera frugiperda Smith (Lepidoptera: Noctuidae), is a transboundary migratory insect pest of agricultural importance. In early 2019, FAW was reported in Jiangcheng County, Yunnan Province, China, and it could seriously threaten the security of China's food crops. Biological control is one of the long-term effective control strategies for FAW. It is necessary to evaluate local natural enemies due to the absence of its natural biological control agents in our country. P. zhonghuajia can parasitize all stages of FAW, which may cause the death of host by interfering host development and suppressing its immune response. This project will conduct experiments on the parasitic selectivity of P. zhonghuajia against FAW, the lethal effects of P. zhonghuajia on FAW, immunosuppressive effects of P. zhonghuajia on FAW, and effects of P. zhonghuajia parasitism on the transcription level of FAW. The results of this project will provide great scientific insights in clarifying the parasitic effect of P. zhonghuajia on FAW, acquire the key genes of development and immune response of FAW related with the parasitic lethality of P. zhonghuajia, and reveal the lethal mechanism of P. zhonghuajia on FAW. These findings will provide scientific basis for preventing and controlling FAW in China.