抗菌肽@ZIF-8纳米粒联合阿魏菇多糖治疗宫颈癌的效果及作用机理

宫颈癌是常见的女性恶性肿瘤之一，严重威胁着全球女性的健康，目前缺乏有效的治疗药物和方法。新疆家蚕抗菌肽具有多种药理作用，对多种恶性肿瘤均表现出优异的抗肿瘤活性。本项目以新疆家蚕抗菌肽既可直接杀伤肿瘤细胞，又可激活体内DNA损伤反应，增强宿主抗肿瘤免疫能力的独特抗肿瘤机理为基础，辅以ZIF-8纳米材料的结构优势，构建抗菌肽@ZIF-8纳米载药体系，研究抗菌肽@ZIF-8纳米粒体内外对宫颈癌细胞生长的抑制作用及其与肿瘤细胞DNA的相互作用和诱导DNA损伤反应的作用机理。同时，结合新疆特有的药食两用真菌阿魏菇中分离提取的阿魏菇多糖作为安全、高效的免疫调节剂，联合用药治疗U14宫颈癌模型小鼠，探讨新疆家蚕抗菌肽@ZIF-8纳米粒与阿魏菇多糖联用对宫颈癌的协同治疗效果及作用机理。本研究为宫颈癌治疗提供新型有效的治疗药物和治疗策略，为新疆特色动植物资源的开发利用提供新的研究思路。

Cervical cancer is one of the common female malignant tumors, which seriously threatens the health of women all over the world. At present, there is a lack of effective treatment drugs and methods. The antibacterial peptide of Xinjiang silkworm has many pharmacological actions and has excellent antitumor activities against many kinds of malignant tumors. This research is based on the unique anti-tumor mechanism that Xinjiang silkworm antibacterial peptide can not only kill tumor cells directly, but also activate DNA damage reaction in vivo to enhance host anti-tumor immunity, supplemented by the structural advantages of zif-8 nano materials, to construct the antibacterial peptide @ zif-8 nano drug delivery system. The inhibitory effect of antibacterial peptide @ zif-8 nanoparticles on the growth of cervical cancer cells in vitro and in vivo, and the mechanism of its interaction with DNA and inducing DNA damage reaction were elucidated. At the same time, the polysaccharide extracted from Pleurotus ferulae, a special edible and medicinal fungus in Xinjiang, was used as a safe and efficient immunomodulator to treat U14 cervical cancer model mice, to investigated the synergistic therapeutic effect and mechanism of antibacterial peptide @ zif-8 nanoparticles combined with Pleurotus ferulae polysaccharide. This study provides effective drugs and treatment strategies for the treatment of cervical cancer, and provides new ideas for the development and utilization of animal and plant resources in Xinjiang.