基于LF-NMR/MRI、TA等多维表征中药炮制“少泡多润，药透水尽”科学内㴠的研究

饮片是体现中医疗效的根本手段，软化是饮片加工的关键。软化程度判断依赖于老药工的眼看、手试、口尝等“看水头”感观经验，导致饮片质量优劣有别、批间迥异，亟需建立客观量化的评价指标与方法。本课题在白芍软化机理初步探明的基础上，聚焦软化传统经验术语传承差的共性问题，选择大黄、黄柏等常用品种为模型，以软化表征方法缺失和机理不明为问题导向，以老药工经验为纽带，集成质构仪、LF-NMR/MRI、HPLC、电镜等技术手段，从宏观的质构性质和组织结构、微观的水分迁移和化学成分等方面为突破口，创新性提出以质构参数等数据为量化的软化终点判断指标，构建客观的软化程度判断方法，利用统计学进行相关性和影响规律分析，基于图像和数字等揭示软化机理，建立软化终点快速判断方法，实现药材软化判断由经验向客观、科学化表征转变，阐明药材软化“少泡多润、药透水尽”科学内涵，对守正中药炮制经验、创新炮制评价方法具有重要意义和应用价值。

Chinese herbal slices are the fundamental means of embodying the effectiveness of traditional Chinese medicine. Softening is the key to processing Chinese herbal slices. Judging the degree of softening depends on the experience of " Kan Shui Tou " of old pharmaceutical workers, such as seeing, hand-testing, and mouth-tasting. This results in the quality of the Chinese herbal slices being different and different from batch to batch. It is urgent to establish objective and quantitative evaluation indicators and methods. This project is based on the preliminary identification of the softening mechanism of Paeonia lactiflora, focusing on the common problems of softening the inheritance of traditional empirical terminology, selecting common varieties such as rhubarb and cork as models, and taking the lack of softening characterization methods and unknown mechanisms as the key issues. The experience of veteran pharmaceutical workers is a bond, integrating texture instruments, LF-NMR / MRI, HPLC, and electron microscopy, and other technical means. It breaks through from the aspects of macroscopic texture and structure, microscopic water migration and chemical composition, and proposes innovatively. Using the data such as texture parameters as the quantified softening end point judgment index, construct an objective softening degree determination method, use statistics to analyze the correlation and influence rules, reveal the softening mechanism based on images and numbers,and establish a fast softening end point judgment method to achieve The judgment of softening of medicinal materials has changed from experience to objective and scientific characterization, and clarified the scientific connotation of softening of medicinal materials, " Shao Pao Duo Run ,Yao Tou Shui Jin ", which is of great significance and application value for basing on traditional Chinese medicine processing experience and innovative evaluation methods.