热激转录因子Ff-HSF1介导CO2调控金针菇菌盖开伞的分子机制

菌盖是大多数食用菌子实体的重要组成部分，通常把菌盖的发育、变大和展开过程称为菌盖开伞。CO2是调控食用菌菌盖开伞速度、决定子实体品质好坏的关键环境因子；然而CO2对菌盖开伞的调控机制并不清楚，食用菌栽培的CO2参数控制仍凭经验。我们前期的研究发现，热激转录因子Ff-HSF1参与高浓度CO2对金针菇菌盖开伞的负调控，但具体分子机制还未被揭示。本项目拟以主栽食用菌“金针菇”为模式材料开展以下研究：1）采用过表达、干扰等方法研究Ff-HSF1在CO2调控金针菇菌盖开伞过程的具体功能；2）基于染色质免疫共沉淀测序、转录组测序、凝胶迁移实验等技术，鉴定Ff-HSF1下游靶标基因，构建Ff-HSF1介导的下游基因调控网络；以期揭示Ff-HSF1介导下CO2调控菌盖开伞的分子机制，为金针菇生产的CO2浓度精细化调控提供理论依据，助力食用菌栽培管理技术革新。

The pileus is an important part of most edible mushroom fruiting bodies. The processes of pileus developing, enlarging and unfolding are collectively called as pileus expansion. Carbon dioxide is a key environmental factor that regulates the expansion speed of pileus and determines the quality of mushroom fruiting bodies. However, the regulation mechanism of CO2 on pileus expansion remains unclear, and the control of CO2 parameters only based on experience during edible mushroom cultivation. Our previous study suggested that the heat shock transcription factor Ff-HSF1 played a negative role in regulation of pileus expansion when response to high CO2 concentration, while the molecular mechanism has not been studied. In order to further investigate the roles of FF-HSF1 in regulating pileus expansion process of the main cultivated edible mushroom “Flammulina filiformis” in response to CO2, gene overexpression and RNA interference will be performed. Then ChIP-seq, RNA-seq and EMSA strategies will be applied to identify the gene regulatory network of FF-HSF1 and further reveal the molecular mechanism of heat shock transcription factor Ff-HSF1 in regulating F. filiformis pileus expansion mediated by CO2, and promote the technical innovation of edible mushroom cultivation.