新型自收缩植骨胶带协同调控YAP促进牙槽突裂微应力感应成骨的应用及机制研究

牙槽突裂是常见的先天颌面部缺损疾病。现阶段的牙槽突裂植骨术面临着患儿创口完善关闭困难、移植骨与缺损部位难以紧密结合、缺损区原位成骨活性不足的挑战，导致植骨区域骨创封闭不佳、成骨感应迟钝、成骨动力缺乏。针对牙槽突裂植骨术的临床难题，本项目拟制备一种自收缩生物胶带用于牙槽突裂植骨术，利用胶带中的儿茶酚基团实现术区的粘接封闭；以热感自收缩电纺纤维持续释放微应力激活成骨感应；同时依靠复合微球材料内源性构建富镁环境，进一步放大成骨动力，协同上调YAP促进植骨术区微应力感应成骨。本项目拟筛选制备参数优化胶带性能；以体内外牙槽突裂植骨模拟实验探究材料的成骨效应；通过表征YAP、ITG和GSN等节点蛋白的表达水平，察明材料在基因层面对应力感应成骨的协同激活作用，并探究其成骨传导机制。期望该胶带能突破牙槽突裂植骨术的临床瓶颈，保障牙槽突裂的骨修复效果。

Alveolar cleft is a common congenital maxillofacial defect disease. The current bone grafting for alveolar cleft is obstructed with the difficulty of wound’s complete closure, slack combining between the graft and the defect bone, insufficient in-situ osteogenic activity of the cleft area, resulting in poor closure, weak osteogenesis -induction and osteogenesis motion. Aiming at the clinical problems of alveolar cleft bone grafting, this project intends to prepare a self-shrinking biotape for alveolar, using the catechol modified tape to realize the bonding and sealing of the operation alveolar; Thermal shrinkage electrospun fibers continue to auto-shrink to activate osteogenic induction; at the same time, relying on the composite microsphere building an Mg2+-rich environment endogenously, thus further amplifies the osteogenesis dynamics, all the components synergistically increases YAP to promote micro-stress-induced osteogenesis in the bone grafting area. This project intends to screen the component parameters to optimize the physical and chemical properties of the tape; through in vivo and in vitro alveolar cleft bone grafting simulation experiments, explore the osteogenic effect of the material; using the characterization of node proteins such as YAP, ITG and GSN to verified the mechanism which the noval tape using to synergistically promote osteogenesis and explore its osteogenic conduction pathway. It is expected that the tape could break through the clinical bottleneck of alveolar cleft bone grafting and ensure its bone repair effect.