基于RAD-seq的鼠尾草属丹参组的系统学研究与分类修订

丹参组曾隶属于鼠尾草属荔枝草亚属，共有19种3变种。最近，丹参组被置于新界定的东亚鼠尾草亚属中，原丹参组中有5个种被转移至其它组，现丹参组共计13种、3变种和1存疑种。该组植物是东亚鼠尾草亚属中药用价值最大的一个类群，除长冠鼠尾草外，其余种类全部为中国特有种。然而，丹参组存在物种界限模糊、部分种类的分类和系统地位不明确等问题。目前的形态学和基于有限DNA片段的分子系统学研究都未能解决以上，给该组物种的实际应用带来极大的麻烦。本项目将利用简化基因组测序技术RAD-seq，在居群水平对丹参组物种开展群体主成分分析、遗传结构分析和系统发育分析，结合形态学证据，拟阐明丹参复合群的物种界限，澄清美丽鼠尾草的物种地位，解决长冠鼠尾草支系的系统位置。本研究结果可为合理开发利用丹参组物种资源提供理论支持，也可为鼠尾草属其他支系的系统学研究和形态近缘种的分类界定提供参考。

Salvia sect. Drymosphace had been placed in subg. Sclarea, including 19 species and three varieties. Recently, the section was placed in the newly defined subg. Glutinaria, consisting of 13 species, three varieties and one doubtful species, with five species being removed. Taxa of sect. Drymosphace are the most important medicinal plants of the subg. Glutinaria. Except for S. plectranthoides, the rest taxa are endemic to China. However, there are lots of problems such as blurred species boundaries and unclear taxonomic and phylogenetic relationships for some species within sect. Drymosphace. At present, morphological and molecular phylogenetic studies based on limited DNA markers fail to solve these problems, which brings great trouble to the practical utilization for species of this section. Based on population-level sampling and RAD-seq, this project will reconstruct phylogenetic tree, together with performing principal component analysis and population genetic structure analysis using SNP data. Combining with morphological analysis, we will clarify the species boundaries of the Salvia miltiorrhiza complex, identity of S. meilienis, phylogenetic position of Salvia plectranthoides Group. This study will provide theoretical support for utilization of taxa of sect. Drymosphace, and also provide a case for the phylogenetic study of other clades of Salvia and species delimitation of morphologically related species.