基于ICAM-1抗体靶向超声微泡构建基因精准递送体系在周围神经损伤分子成像及治疗中的研究

周围神经损伤的基因治疗是神经再生研究中的重要方向之一。如何促使基因高效、精准递送至神经损伤部位并同时模拟神经再生微环境，加速再生神经与靶器官对接，是该领域亟待解决的问题。周围神经损伤的基因治疗主要分为两个过程，即精准定位神经损伤部位；基于精准定位，利用靶向基因递送体系治疗损伤节段。本项目在前期研究工作基础上，①利用周围神经损伤后ICAM-1在血管内皮细胞膜表面的高表达作为特异性靶点，构建负载ICAM-1的超声靶向微泡，在分子成像水平精准定位神经损伤部位；②以富血小板血浆外泌体作为基因载体结合超声靶向微泡破坏技术，构建外泌体携基因精准递送体系，提高神经再生效率，为周围神经损伤修复提供新的非手术治疗策略。

Gene therapy of peripheral nerve injury is one of the important directions in nerve regeneration research. The way to promote the efficient and accurate delivery of genes to the injured part of the nerve, while simulating the microenvironment of nerve regeneration to promote the docking of the regenerated nerve with the target organ is an urgent problem to be solved in this field. Gene therapy of peripheral nerve injury is mainly divided into two processes. After precisely locating the nerve injury site, a targeted gene delivery system is used to treat damaged segments based on the precise positioning. With the previous research work done, this project include two research purposes. The one is using the high expression of ICAM-1 as a specific target on the surface of the vascular endothelial cell membrane after peripheral nerve injury to construct ultrasound targeted microbubbles loaded with ICAM-1 to accurately locate the nerve injury site at the molecular imaging level; The other one is using platelet-rich plasma exosomes as a gene carrier combined with ultrasound targeted microbubble destruction technology to construct a precise gene delivery system for exosomes to improve the efficiency of nerve regeneration.