褐飞虱ILP2响应丼冈霉素诱导水稻糖含量增加调控生殖增长的作用机制

褐飞虱是一种典型的农药诱导的再猖獗重大害虫。以前研究表明褐飞虱取食井冈霉素喷雾处理的水稻导致其雌虫体内生化物质改变和脂肪代谢增强，刺激褐飞虱生殖。但其作用机制不清楚。最近研究发现JGM喷雾处理可诱导水稻体内蔗糖和葡萄糖含量显著增加，导致取食该水稻的褐飞虱雌虫ILP2上调表达和激活TOR通路。基于果蝇和其它昆虫ILPs对糖营养因子的感知机制，我们提出“褐飞虱ILP2响应JGM诱导水稻糖含量增加激活TOR通路调控生殖增长”的科学假设。本项目拟在前期研究的基础上采用RNAi干扰、生物测定、Western blot 和免疫荧光等方法，阐明褐飞虱ILP2响应JGM诱导水稻糖含量增加激活TOR通路的机制，解析ILP2/TOR通路响应水稻糖含量增加调控褐飞虱生殖增长的作用机理，最终从胰岛素感知营养因子变化角度揭示JGM刺激褐飞虱生殖的分子机制。该研究结果将为褐飞虱开发新的农药靶标及抗性治理提供新的思路。

The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) is a typical resurgent pest induced by pesticides. Previous studies showed that BPH feeding on rice plants sprayed with jinggangmycin (JGM) resulted in changes in biochemical substances and enhancing fat metabolism in BPH female, stimulating BPH reproduction. However, its regulatory mechanism remains unclear. The recent studies showed that the glucose and sucrose contents was significantly increased in the rice plants sprayed with jinggangmycin (JGM), a fungicide widely used in China for controlling rice sheath blight, Rhizoctonia solani, compared to control rice plants, resulting in the up-expression of insulin-like peptide 2 (ILP2) and activation of the target of rapamycin (TOR) signaling pathway in BPH female after feeding on rice plants sprayed with JGM. Based on the reported roles of ILPs in perception and regulation of sugar nutrition in Drosophila melanogaster and other insects, we proposed the scientific hypothesis that BPH ILP2 in response to JGM-induced increase in sugar content of rice plants regulates reproductive growth by activing the TOR signaling transduction pathway. Based on our previous accumulated achievements, this project aims to test this hypothesis using RNAi interference, bioassay, western blot and immunofluorescence approaches. Firstly, we intend to unravel the mechanism of ILP2 in BPH female triggers the TOR signaling pathway in response to JGM-induced increase sugar content of rice plants; secondly, we want to reveal the mechanism of the ILP2/TOR signaling pathway regulates BPH reproductive growth in response to JGM-induced increase sugar content in rice plants. By comprehensive analysis of results derived from this project, the molecular mechanism of JGM stimulated BPH fecundity from the perspective of BPH insulin sensing changes in sugar nutrition of will be illustrated. The results will provide new ideas for the development of new pesticide targets and resistance management in BPH.