水稻齿叶矮缩病毒Pns6蛋白与寄主因子互作的分子机制研究

近年来，伴随着SRBSDV的爆发，由RRSV引发的水稻矮缩病危害明显加重。前期研究发现，RRSV 编码的Pns6蛋白能够显著促进异源病毒（SRBSDV和PVX）的复制，却抑制同源病毒RRSV的复制，说明Pns6在调控RRSV致病及SRBSDV/RRSV协生互作中起重要作用；然而，酵母双杂交实验发现Pns6与SRBSDV或RRSV编码的各蛋白均不存在互作，故推测Pns6蛋白的这种“利他损己”调控病毒复制的行为是通过与寄主因子互作来实现的。本项目拟利用蛋白质互作研究等技术筛选鉴定与Pns6蛋白互作的水稻寄主因子，鉴定Pns6与寄主因子互作的结构域，明确其亚细胞定位，分析寄主因子在病毒复制中的作用，最终阐明RRSV Pns6蛋白与寄主因子互作的分子机制。研究结果为解析RRSV的致病机制及深入研究SRBSDV/RRSV协生互作的分子机制打下基础，同时为利用寄主因子防控水稻病毒病提供理论支撑。

In recent years, with the outbreak of Southern rice black-streaked dwarf virus(SRBSDV), the occurance of rice dwarf disease caused by Rice ragged stunt virus(RRSV) has shown a significant increasing trend. Previous studies have found that the Pns6 protein encoded by RRSV can significantly promote the replication of heterologous viruses (SRBSDV and PVX), but inhibit the replication of homologous virus (RRSV), indicating that Pns6 plays an important role in SRBSDV/RRSV synergistic interaction and regulation of RRSV pathogenesis. However, yeast two-hybrid experiment found that Pns6 had no interaction with the proteins encoded either by SRBSDV or RRSV, suggesting Pns6 probably regulate viruses replication by interaction with the rice host factors. This project is conducted to elucidate the mechanism for this phenomenon: protein interaction technologies were applied for screening and identifiing RRSV Pns6 protein interaction host factors. and the interaction domains and subcellular locations of Pns6 and host factors were identified , the function of host factors on virus infection and symptom development were explored. Finally elucidate the molecular mechanism of interaction between RRSV Pns6 protein and rice host factors. Results of this project will lay a foundation for elucidating molecular mechanism of RRSV-rice intaction and elucidating the molecular mechanism of SRBSDV/RRSV synergistic interaction, and will provid a theoretical base for preventing and controling rice viral diseases by using host factors.