基于RVLM区RAS双轴及其下游通路介导的神经炎症与交感神经兴奋探讨从阳明论治高血压的中枢机制

高血压是危害人类健康的重大公共卫生问题。前期研究证实从阳明论治高血压疗效确切，其降压效应可能与中枢响应特征改变有关，但具体机制尚不明确。最新研究表明RVLM区RAS双轴失衡介导的神经炎症和交感神经兴奋，是高血压发病的重要机制。既往研究发现电针阳明经穴可改善神经炎症，抑制交感神经兴奋，发挥降压作用。据此，提出如下假说：电针阳明经穴通过调控RVLM区RAS双轴[ACE-AngII-AT1R与ACE2-Ang(1-7)-MasR]及下游MAPK和PI3K通路，改善神经炎症，抑制交感神经兴奋，发挥降压效应。本研究拟从RVLM区RAS双轴及下游通路入手，以神经炎症和交感神经兴奋为切入点，运用无线遥测、免疫荧光、蛋白免疫印迹等方法，通过动物和细胞实验从正、反两面研究电针阳明经穴对血压、交感神经兴奋性、神经炎症、RAS双轴及下游通路的影响，揭示电针阳明经穴降压的中枢机制，为从阳明论治高血压提供科学依据。

Hypertension has seriously endangered people's health, and has become a major public healthy and social problems. Our previous clinical study demonstrated that the treatment for hypertension from Yang Ming meridian had evident antihypertensive effect, and the antihypertensive effect may be related with the change of characteristics of central response. Recent evidence reveals that the dynamic imbalance between the mutual antagonism axes of RAS system [ACE-AngII-AT1R axis and ACE2-Ang(1-7)-MasR axis] leads to neuroinflammation and sympathetic excitability closely related to development of hypertension. Previous studies has confirmed that electro-acupuncturing on acupoints of Yang Ming meridian can relieve the neuroinflammation, decrease sympathetic nerve excitation and lower blood pressure. Therefore, we hypothesized that electro-acupuncturing on Yang Ming acupoints may reduce neuroinflammation and attenuate sympathetic excitability by regulating the ACE-AngII-AT1R axis and ACE2-Ang(1-7)-MasR axis and PI3K and MAPK pathways, then lower the blood pressure. In this study, we will use technologies like wireless telemetry, immunofluorescence, western-blotting et al. By means of in vivo experiment and in vitro experiment, we try to identify the effect of electro-acupuncturing on Yang Ming acupoints for blood pressure, neuroinflammation and sympathetic nerve excitation, RAS axes and their downstream pathways (MAPK pathway and PI3K pathway). By revealing its central mechanism, we intend to provide scientific theory for treatment of hypertension with Yang Ming meridian.