胡杨PeRIN4-REM6.5-HA1蛋白互作网络调控质子泵活性及离子平衡分子机制研究

胡杨质膜（PM）H+-ATPase在盐胁迫响应及离子平衡调控过程中发挥了极其关键的作用。前期从转录调控的角度研究胡杨PM H+-ATPase基因（PeHA1）的盐诱导表达，发现了转录调节因子调控PeHA1表达的PeHSF-WRKY1-HA1信号网络。在此基础上，本项目拟从翻译后蛋白-蛋白互作角度进一步研究胡杨PM H+-ATPase活性的调控机制，重点探讨胡杨RPM 1-interacting protein 4（RIN4）、Remorin（REM）蛋白与PeHA1的相互作用。植物RIN4与REM蛋白在生物防御反应中发挥重要作用，但RIN4与REM在植物适应盐胁迫中的作用机制并不明确。本项目重点探讨胡杨PeRIN4、PeREM6.5、PeHA1蛋白互作调控质子泵活性的分子机理，建立胡杨离子平衡调控的PeRIN4-REM6.5-HA1蛋白互作网络，深入阐明耐盐树木响应盐胁迫的信号转导途径。

The plasma membrane (PM) H+-ATPases are critical for triggering salt signals and maintaining K+/Na+ homeostasis in Populus euphratica under NaCl salinity. We have previously investigated the transcription factors-regulated expression of a PM H+-ATPase gene, PeHA1, under salt stress. The transcriptional regulation network of PeHA1, i.e., PeHSF-WRKY1-HA1, was established in our previous study. Based on this work, we plan to investigate the protein-protein interaction network in the post-translational modulation of H+-pumping activity in NaCl-stressed Populus euphratica. The protein interactions among RPM 1-interacting protein 4 (RIN4), Remorin (REM), and PeHA1 will be testified. RIN4 and REM play crucial roles in plant defense to biotic stress. However, the role of RIN4 and REMs in plant response to salt stress is unclear. We will focus on protein interactions among P. euphratica PeRIN4, PeREM6.5, and PeHA1, and the relevance to H+-pumping activity modulations. The objective is to elucidate the role of protein interaction network, PeRIN4-REM6.5-HA1, in the control of H+-pumping activity and K+/Na+ homeostasis under salt stress. The results can add new knowledge to explore the regulation mechanism of PM H+-ATPase and salt signaling pathway in the salt-resistant model tree species.