朋友还是敌人?越橘毛根中杜鹃花类菌根真菌与深色有隔内生真菌互作机制解析

对越橘杜鹃花类菌根真菌(EMF)、深色有隔内生真菌(DSE)进行资源调查、分离及鉴定。分析离体培养代谢产物对EMF、DSE互作及菌丝生长的影响。利用分泌蛋白质组学技术解析不同培养体系中EMF、DSE的分泌蛋白特点。分析接种EMF、DSE越橘根系次生代谢产物的变化，了解其对真菌定殖及越橘生长的影响。基于宏转录组学及全长转录组学技术，深度挖掘不同接种处理中越橘、EMF、DSE的差异表达基因，解析EMF、DSE侵染过程中越橘、EMF、DSE三者基因互作网络及机制。利用同位素示踪及分根培养技术，分析不同接种处理中光合产物、N、P在植物及EMF、DSE菌丝间的分配特点。了解不同营养、环境因子对EMF、DSE定殖、互作及越橘生长的影响。解析EMF、DSE的互作机制，可能让我们对菌根真菌与内生真菌的互作机制有一个全方位的认识；对维持越橘根际生态系统稳定性、提高越橘生产能力也具有重要意义。

Ericoid mycorrhizal fungi (EMF) and dark septate endophytes (DSE) from Vaccinium sp. are surveyed, isolated and identified. We will identify the metabolite of EMF and DSE in in vitro culture, and elucidate the effect on hyphal growth and interaction of EMF and DSE. The characteristic of secretory proteins in different culture systems is analyzed by the secretion proteome methods. We will screen the difference of secondary metabolites of Vaccinium sp. roots inoculated by EMF and DSE, and analyze the effect on fungal colonization and Vaccinium sp. development. Based on metatranscriptomics and full length transcriptomic techniques, we will unearth deeply differential expression genes of Vaccinium sp., EMF, and DSE within different inoculation treatments. Finally, we will elucidate the gene interaction network and mechanism of Vaccinium sp., EMF, and DSE during the invading process of EMF, and DSE. Using isotopic tracer and split root culture methods, the allocation characteristic of photosynthate, N, and P within plants and hypha, is analyzed for different inoculation treatments. Additionally, the different nutrition and environment factors, which have effect on colonization, interaction of EMF and DSE, and Vaccinium sp. development, will be studied. We will possibly understand the interactional mechanism between mycorrhizal fungi and endophytic fungi in a full view via elucidating the interactional mechanism between EMF and DSE. It is significant to improve the production of Vaccinium sp. and keep the stability of ecosystem in rhizosphere.