益智颗粒有效组分（TS）对快速老化痴呆模型小鼠SAMP8大脑β淀粉样蛋白代谢通路影响的研究

益智颗粒（国食健字G20120602）是新开发的对老年性痴呆具有预防保健作用的中药保健产品，动物实验与临床观察均表明其具有改善记忆功能。前期研究发现益智颗粒有效组分（The Effective components，TS）对快速老化痴呆模型小鼠SAMP8记忆障碍具有明显改善作用，同时可抑制SAMP8脑内β淀粉样蛋白（Aβ40与Aβ42）及APP的表达，但其作用机理仍不清楚。本课题将以SAMP8小鼠为研究对象，采用直接荧光法、免疫组化、western blot和定量PCR等方法检测TS对SAMP8小鼠大脑β淀粉样蛋白代谢通路中NF-κB转录因子 P50、α-分泌酶ADAM10、β-分泌酶BACE1和γ-分泌酶PS1、Aph-1等基因蛋白表达及酶活性的影响，弄清TS影响Aβ形成代谢通路的靶位点，阐明TS抗老年性痴呆作用的分子机制，为将其开发成治疗老年性痴呆症国家中药5类新药提供科学依椐。

Yizhi Granules is a new health food with the function of prevention and healthcare, and available for the treatment of Alzheimer's disease (AD). The Effective components (TS) is the effective part of Yizhi Granules . Our preliminary experiments showed that TS has obvious effect on the memory improvement in rapid aging dementia SAMP8 mice model and it inhibits the expression of APP、Aβ40 and Aβ42 in SAMP8 brain. However, the mechanism unclear. With the rapid aging dementia SAMP8 mice for model, we will observe the influence of TS on transcription factors NF- κB which directly effects the generation of APP, and the level of secretase which related to Aβ and effect the catabolism of APP, finally make clear the action part and link of TS to affect the metabolic pathways of Aβ, illuminate the mechanism of TS on inhibiting Aβ and it’s precursor protein expression, provide scientific noted for the development of new 5th categories national drugs to treat Alzheimer's disease.