3D多孔碳纳米纤维BPCNFs@ROS/RNS合酶抗氧化探针的可控构建及在肌腱炎症早期损伤中的作用及机制研究

肌腱病是目前常见的一类运动损伤性疾病，且治疗方法有限，愈后效果差，早期阶段的诊断和治疗一直是运动医学的研究热点和难点。肌腱微损伤节点的判断是防治肌腱损伤的关键问题。诸多证据表明，分子炎症中的炎症氧化因子ROS/RNS在肌腱微损伤发展中起着重要作用，但是实现肌腱损伤微环境中的ROS/RNS的精准检测的方法依然异常匮乏，且其内在机制仍尚不清楚。因此，本项目拟构建孔径精准可调的高灵敏性3D多孔碳纳米纤维BPCNFs@ROS/RNS合酶抗氧化探针，明确光纤探针对不同类型的酶的捕获机制；利用功能化探针实现跟腱微损伤大鼠模型和巨噬细胞炎症微环境的H2O2、O2-和NO等氧化因子浓度的高效安全诊断，并建立肌腱微损伤进程与其浓度的关联模型，并深入研究其损伤机理。本项目将为肌腱微损伤的“自查”和“自愈”提供新的模型检测评估手段，以期为临床预防和诊断肌腱损伤提供新的理论依据和技术参考。

Tendinopathy is a common type of sports injury disease at present, and the treatment methods are limited, and the healing effect is poor. Early diagnosis and treatment have always been the research hotspots and difficulties of sports medicine. The judgment of the micro-injury node of the tendon is the key issue to prevent and treat the injury of the tendon. A lot of evidence shows that the inflammatory oxidative factor ROS/RNS in molecular inflammation plays an essential role in the development of tendon microinjury, but the method to achieve accurate detection of ROS/RNS in the microenvironment of tendon injury is still abnormally scarce, and its internal mechanism is still not clear. Therefore, this project intends to construct a highly sensitive 3D porous carbon nanofiber BPCNFs@ROS/RNS synthase antioxidant probe with precise and adjustable pore size; and to clarify the capture mechanism of different types of enzymes by the fiber probe; and to use the functionalized probe to achieve efficient and safe diagnosis of the concentration of oxidative factors such as H2O2、O2- and NO in the micro-injury rat with achilles tendon microinjury model and macrophage inflammation microenvironment, and to establish the correlation model of the tendon micro-injury process and concentration of oxidative factors, and to study its injury mechanism deeply. This project will provide novel model detection and evaluation methods for the "self-examination" and "self-healing" of tendon micro-injury, in order to provide new theoretical basis and technical reference for clinical prevention and diagnosis of tendon injury.