基于“性-效-物”的江西道地药材栀子质量标志物的研究

现行栀子质量标准的控制指标与其功效关联不强，专属性差，不能区分生品及各炮制品、成熟度及同属药材。“药性”与“药效”是中药的“物质基础”作用于人体疾病的不同层面、不同方式的生物效应表达形式，因此基于“性-效-物”的质量标志物是反映中药安全和有效的标示性物质。在前期20余年研究的基础上，本课题以江西道地药材栀子为例，根据性味可拆分，采用系统化学分离及UPLC-Q-TOF/MS对栀子进行物质群的拆分及成分鉴定，通过能量代谢、物质代谢等研究栀子的“寒性”，通过电子舌技术、分子对接技术、G蛋白偶联受体研究栀子的“苦味”，以解热、利胆、抗炎为指标进行谱-效研究，通过药动学、代谢组学及体内过程阐明栀子“功效”的物质基础及作用机制，以最小二乘法、主成分分析等分析“性-效-物”之间的相关性规律，最终确立映反映栀子安全性、有效性的质量标志物，建立多元质量评价方法，为体现中医药自身特点的质量标准提供新模式。

The control components of the current quality standards of Gardeniae Fructus are not strongly related to their effectiveness. The current quality standards of Gardeniae Fructus cannot effectively distinguish Gardeniae Fructus and its processed products, judge the maturity of the fruit, and make a distinction of fruits of Gardenia. "Property" and "response" are the biological effect expression on the "material basis" of traditional Chinese medicine acting on different levels and different ways. Therefore, the quality markers based on “property-response-component” correlation reflect the safety and the effectiveness of traditional Chinese medicine. Gardeniae Fructus have been studied by us more than 20 years. The objective characterization of “cold”is based on systematic chemical separation, UPLC-Q-TOF/MS, energy and substance metabolism, and nervous and endocrine system. The objective characterization of “bitter-taste” is based on electronic tongue technology, molecular docking technology, and G protein-coupled receptors. Spectrum-effect relationship of antipyretic effect, choleretic effect, and anti-inflammation were established. The major effective components and the mechanism were clarified through pharmacokinetics, metabolomics, and in vivo processes. At last, Quality markers were found by correlation analysis of “property-response-component”. The quality control methods of multi-component assaying and fingerprint will been established. Overall, these results will provide a new model for thequality standards that reflect the characteristics of traditional Chinese medicine.