基于双轴离心式微流控的多指标免疫分析技术研究

免疫检测是体外诊断技术的重要组成部分，在日常生活中亦有广泛应用。以肿瘤为代表的慢性疾病和以急性心肌梗死为代表的急性疾病对便携、自动化、多指标的免疫检测技术均有巨大需求，而现有高通量自动化免疫分析仪和侧向流免疫分析设备无法充分满足这些检测需求。基于微流控技术的免疫分析系统能满足便携、自动化的免疫检测需求，但现有系统实现多指标的方式往往靠免疫微阵列技术，使得抗体之间的交叉反应难以避免。.为解决微流控免疫分析中的这一关键难题，本项目提出了一种基于双轴离心式微流控的免疫分析新技术，其利用双轴离心式微流控平台来简化芯片结构，采用以角度控制为核心的多腔体、多步流体操控来自动化实现免疫检测，从根本上避免了不同抗体之间的交叉反应。该技术的特色在于利用简单的微流控芯片结构和简易的硬件设备实现复杂的流体操控，结合芯片试剂存储技术，可实现“样品入-结果出”式全集成、多指标免疫检测。

Immunoassay is an important part of in-vitro diagnostics and widely used in our daily life, especially in health care applications. Recently, the demand of the automated and multiplexed immunoassay for point-of-care (POC) applications has been increasing rapidly in diagnoses of diseases, including chronic diseases such as tumors and acute diseases such as heart attack. However, the current high-throughput automated immuno-analyzers or lateral flow immunoassay products cannot provide POC diagnoses immediately or conveniently. Though microfluidic devices can realize the portable and automatic immunoassay, it is still too tough to perform multiplex detection especially for the multi-step immunoassays. More importantly, protein microarray can deal with the multiple targets, but the cross-reactivity of antibodies cannot be eliminated. .To solve the above problem, a newly designed immunoassay device is to be constructed in this project for the detection of multiple targets on a centrifugal disc. Based on our previously established centrifugal microfluidic platform with two rotation axes, a microfluidic disc was designed for the automation of the immunoassay. Multiple chambers were configured in the disc for the detection of multiple targets. One major advantage of our system is that the centrifugal microfluidic platform with two rotation axes has the capability of handling complex liquid manipulation even for multiple chambers on the disc. The other advantage is that multiple chambers can avoid the cross-reactivity during the immunoassays. The system we are aiming to construct has the great potential to achieve the fully integrated immunoassays in a “sample-in and answer-out” manner for the detection of multiple targets.