LiP8噬菌体侵染单增李斯特菌过程中互作机制的研究

单增李斯特菌（LM）对人类健康、公共卫生安全及畜牧业发展造成了严重威胁，利用噬菌体有望解决细菌耐药性、食品污染等诸多问题。然而，由于噬菌体高度的宿主特异性，以及与LM之间相互作用的研究较少，使得噬菌体的应用受到很大限制。因此，在前期工作基础上，本研究围绕噬菌体入侵LM时与宿主菌互作机制，通过采用酶功能分析、转录组学测序、蛋白分子互作、基因组学测序、细菌改造等方法，重点研究：（1）噬菌体入侵LM时对胞外多糖、细胞壁的降解，以及噬菌体受体结合蛋白与受体分子互作的解析；（2）噬菌体侵染LM后不同时间点，二者在基因转录及表达方面的差异及影响，进而从协同进化的角度分析其作用机制；（3）LM耐受噬菌体感染在基因组学上的分子机制。以上研究对于理解噬菌体侵染LM的机理，探究噬菌体和宿主菌在长期协同进化中的相互关系，指导人们理性、高效防控LM污染食品及所致疾病方面具有科学意义。

Listeria monocytogenes (LM) has posed a serious threat to human health and husbandry development. However, due to the lack of basic research on the interaction between phage and LM, the utilization of phage is greatly limited. By using of enzyme function analysis, transcriptome sequencing, protein molecular interaction analysis, genomics sequencing, bacteria transformation and other methods, we focus on the interaction mechanism between phage and host bacteria when it invade into LM. Importantly, the study include the following: (1)Degradation of exopolysaccharides, cell walls, and interaction between phage receptor binding proteins and receptor molecules during phage invasion of Listeria monocytogenes. (2)The differences and influences of phage infection on gene transcription and expression at different time of LM, even analyzed from the perspective of co-evolution. (3)The molecular mechanism of LM tolerance to phage infection in genomics. This study has scientific significance for understanding the mechanism of phage infection on LM, exploring the mutual relationship between phage and host bacteria in long-term co-evolution, and guiding people to prevent and control LM rationally and efficiently.