住肉孢子虫入侵对藏羊肠道黏膜免疫的作用机制研究

住肉孢子虫病是一种寄生宿主广泛的人兽共患寄生虫病，在动物群体中感染率高，其中在青海高原牧区特有物种--藏羊的体内寄生数量更高，严重影响着地区畜牧业经济发展和人们的饮食健康。该虫体生活史相对复杂，需要两个或多个宿主，侵入动物体内后的致病机理尚不明确。为进一步明确住肉孢子虫在侵入动物体内及移行过程中引起的危害和机体免疫应答机制，本研究在掌握青海藏羊住肉孢子虫流行情况、种属分类及细胞培养方法的基础上，采用OTU法分析虫体对肠道菌群的调节作用，运用ELISA法、PCR法、流式细胞术检测虫体对黏膜免疫机制的作用。通过该项目的系列研究明确住肉孢子虫在侵入藏羊体内及移行过程中引起的危害和机体免疫应答作用机制，为进一步开发生物疫苗奠定基础，同时也为预防藏羊住肉孢子虫病提供一定的理论依据。

Sarcosporidiosis is a zoonotic parasitic disease with a wide range of parasitic hosts. Tibetan sheep as a unique species has a high infection rate in Qinghai plateau pastoral areas, which has a higher number of parasites, seriously affecting the economic development of regional animal husbandry and people's dietary health. The life cycle of the sarcocystis is relatively complex, requiring two or more hosts. And the pathogenic mechanism after invasion into animals is still unclear. To further clarify the harm caused by the invasion and migration of Sarcocysts and the mechanism of immune response, the present study was conducted based on mastering the epidemic situation, species classification and cell culture methods of Sarcocysts in Qinghai Tibetan sheep. The OTU method was used to analyze the regulation of the intestinal flora by sarcocystis. The effects of sarcocystis on mucosal immune mechanisms were detected by ELISA, PCR and flow cytometry. Through a series of studies of this project, the harm and immune response mechanism of sarcocysts caused by its invasion and migration into Tibetan sheep were clarified, which laid a foundation for the further development of biological vaccines and provided a certain theoretical basis for the prevention of sarcosporidiosis in Tibetan sheep.