白菜eIF(iso)4E基因T106C位点对TuMV的抗病机理解析

芜菁花叶病毒（TuMV）是危害白菜的主要病害之一，而eIF(iso)4E是白菜抗TuMV的重要基因。项目申请人前期在白菜中克隆3个eIF(iso)4E基因拷贝，验证了eIF(iso)4E不同拷贝与TuMV不同株系的互作关系，揭示了eIF(iso)4E基因关键SNP位点（T106C）对二者互作的重要影响。为了系统解析白菜eIF(iso)4E基因关键SNP位点（T106C）的抗病机理，项目申请人拟对300份白菜自然群体材料的eIF(iso)4E基因序列进行分析，统计T106C变异在白菜抗/感TuMV中的频率；同时，构建单碱基编辑载体和超表达载体，对白菜eIF(iso)4E基因关键SNP位点（T106C）进行定点编辑和超表达验证。通过RNA-seq对eIF(iso)4E基因的靶标基因进行筛选，进而解析eIF(iso)4E基因关键SNP位点（T106C）对TuMV的抗病机理。

Turnip mosaic virus (TuMV) is one of the main diseases to Chinese cabbage, and eIF(iso)4E is an important gene resistance to TuMV in Chinese cabbage. At the early stage, the project applicant cloned three copies of eIF(iso)4E genes in Chinese cabbage, and verified the interaction between different copies of eIF(iso)4E and various strains of TuMV, revealing the important influence of key SNP locus (T106C) of eIF(iso)4E gene on the interaction between them. In order to systematically analyze the resistance mechanism of the key SNP locus (T106C) of eIF(iso)4E gene in Chinese cabbage, the project applicant planned to analyze the eIF(iso)4E gene sequences from 300 natural population materials of Chinese cabbage, and calculate the frequency of T106C variation in resistance/susceptibility of Chinese cabbage to TuMV. Meanwhile, a single base editing vector and an overexpression vector were constructed for site-specific editing and overexpression verifying of the key SNP site (T106C) of eIF(iso)4E gene in Chinese cabbage. The target genes of eIF(iso)4E gene were screened by RNA-seq, and then the disease resistance mechanism of the key SNP locus of eIF(iso)4E gene (T106C) against TuMV was analyzed.