褐飞虱抗烯啶虫胺细胞色素P450基因CYP6ER1转录调控机制研究

褐飞虱是水稻生产上最为严重的农业害虫。代谢解毒酶基因上调表达是昆虫对杀虫剂产生抗药性的重要途径，且代谢解毒酶基因的转录调控是近年抗药性分子机制研究的热点。前期研究表明细胞色素P450基因CYP6ER1上调表达介导褐飞虱对烯啶虫胺产生抗药性，但褐飞虱抗烯啶虫胺细胞色素P450基因CYP6ER1转录调控的分子机制尚不明确。本项目拟在前期研究基础上，构建细胞色素P450基因CYP6ER1启动子序列及其截短序列重组质粒，分析不同长度启动子活性，明确调控细胞色素P450基因CYP6ER1表达的启动子关键序列区域；鉴定调控细胞色素P450基因CYP6ER1上调表达且响应烯啶虫胺的转录因子；揭示转录因子在调控褐飞虱抗烯啶虫胺中的作用。通过以上研究，阐明褐飞虱抗烯啶虫胺细胞色素P450基因CYP6ER1转录调控的分子机制。研究结果有助于昆虫毒理学理论拓展与创新，为开发害虫抗药性治理新技术提供重要理论依据。

The brown planthopper, Nilaparvata lugens (Stål), is an important pest in rice throughout Asia. The over-expressed of detoxifying enzyme genes is an important factor for insect resistance to insecticide, but determining and elucidating regulation mechanism of these genes is an important task in insecticide resistance research in recent years. And the study of the mechanism of resistance against nitenpyram in N. lugens indicated that the cytochrome P450 CYP6ER1 could confer resistance to nitenpyram in N. lugens. However, the transcriptional regulation of this resistant gene (the cytochrome P450 CYP6ER1) responsible for nitenpyram resistance remains unknown. On the basis of previous studies, construct a series of fragments of different regions promoter region upstream of the cytochrome P450 CYP6ER1, and analyze the promoter activity, and identify its core sequence. Screen the transcription factors that could regulate the expression of CYP6ER1 of the cytochrome P450 and respond to nitenpyram. And elucidate the binding and regulation of transcription factors against the cytochrome P450 CYP6ER1. Reveal the role of transcription factors in the regulation of the cytochrome P450 CYP6ER1 expression associated with the development of resistance to nitenpyram. The results will provide an important theoretical and practical significance for the continuous and effective control of N. lugens. And it also provides the important theoretical basis for developing new and environmentally friendly pest control and resistance control strategies.