类受体胞质激酶RSBs调控bak1 bkk1双重缺失突变体免疫自激活的分子机理

多数高等植物具有固着生长的特性，必须直面生长环境中各种致病微生物的威胁。在长期的适应性生长中，植物逐渐进化出了一类类受体胞质激酶(RLCK)蛋白，在帮助植物抵御病原微生物的免疫调控中发挥重要功能。申请人前期发现当植物受体激酶BAK1及其同源蛋白BKK1同时缺失时，会触发依赖于抗病蛋白ADR1s的免疫自激活。但这些抗病蛋白如何被激活以及激活后又调控了哪些信号组分，是植物学领域的研究热点。我们发现一组功能尚未被揭示的RLCKs，RSBs，可参与调控bak1 bkk1的免疫自激活反应，推断RSBs很可能通过参与新型抗病小体的形成来调控植物免疫反应的激活。本项目旨在利用遗传、生化、细胞生物学等一系例研究手段，进一步探究RSBs在调控植物免疫中的具体作用机理，同时挖掘与RSBs共同行使功能的其他成员并完善RSBs介导的植物免疫调控网络，为未来新型抗病作物的选育及绿色农业可持续发展提供理论参考。

The majority of higher plants are sessile in nature. They have to directly face constant attacks by pathogenic microbes from their living environments. Plants gradually evolved a large group of proteins, named receptor-like cytoplasmic kinases (RLCKs), which play key roles in the defense responses of plants. Our previous studies indicated that knocking out both receptor-like kinase (RLK) BAK1 and its paralog BKK1 can trigger a series of autoimmunity responses and these processes rely on a group of resistance proteins designated as ADR1s. The activation mechanisms of these resistance proteins and the downstream proteins they regulate are largely unknown. These questions have become hot topics in plant biology. We identified a group of functionally undefined RLCKs, named RLCKs suppressing bak1 bkk1 (RSBs), which are involved in bak1 bkk1 associated autoimmunity responses. Our preliminary results suggested that RSBs are likely involved in the activation of immunity response via the formation of resistosome. The aims of our proposal are to investigate the detailed molecular mechanisms of RSBs in mediating plant immunity response and to identify their functionally related other regulatory proteins. These analyses will help us to better understand the RSBs-associated immunity network. These results can be used as references for us to develop genetically modified crops with improved disease resistance in the future.