BrbZIP转录因子介导ABA和光周期调控不结球白菜抽薹开花的分子机理

外源逆境胁迫可导致不结球白菜先期抽薹，严重影响其产量和品质。ABA作为胁迫响应的重要激素，对不结球白菜抽薹开花的调控受光周期的影响。但ABA与光周期的关系还不清楚。前期，我们发现不结球白菜光周期开花途径关键基因BrCO启动子可与一个新的ABA响应转录因子BrbZIP互作，这为研究ABA和光周期的关系提供了新路径。本项目在此基础上，拟通过酵母单杂交、免疫共沉淀与串联质谱、VIGS、遗传转化和染色质免疫共沉淀等技术，研究BrbZIP在不结球白菜中的生物学功能和上游ABA响应途径，明确BrbZIP蛋白与其他蛋白的互作调控关系，解析BrbZIP作用于BrCO调控抽薹开花的信号网络，从而揭示ABA协同光周期调控不结球白菜抽薹开花的分子机理。同时，也对通过改变外界环境来调控不结球白菜抽薹开花时间提供理论指导。

Exogenous stress can lead to premature bolting of non-heading Chinese cabbage, which seriously affects its yield and quality. Abscisic acid (ABA), as an important hormone in stress response, is affected by photoperiod in the regulation of bolting and flowering of non-heading Chinese cabbage. However, the relationship between ABA and photoperiod is not clear. Previously, we found that BrCO promoter, a key gene of photoperiod flowering pathway in non-heading Chinese cabbage, can be bound by a new ABA response transcription factor BrbZIP, which provides a clue for the study of the link between ABA and photoperiod pathways. On the basis in this project, through yeast one hybrid, immunocoprecipitation and tandem mass spectrometry, virus induced gene silencing, genetic transformation and chromatin immunoprecipitation technologies, we intend to study the biological function and upstream ABA response pathway of BrbZIP in non-heading Chinese Cabbage, to clarify the interaction between BrbZIP and its other proteins, to analysis the signal network of BrbZIP binding to BrCO in regulation of bolting and flowering, thereby to reveal the molecular mechanism of ABA cooperating with photoperiod in regulating the bolting and flowering of non-heading Chinese cabbage. Meanwhile, it also provides theoretical guidance to regulate the bolting of non-heading Chinese cabbage by changing the external environment.