基于大豆蛋白颗粒/细菌纤维素模拟脂肪组织构建HIPPEs体系的稳定机制

脂肪模拟物是食品加工中动物脂肪的替代品。目前有关构建类似脂肪组织结构的高内相Pickering乳液（HIPPEs）体系形成与稳定机制还不清晰。本项目在前期对大豆蛋白颗粒（SPP）形成高内相乳液凝胶研究基础上，拟围绕“组装构建-稳定解析-加工评价”思路，明确SPP与细菌纤维素（BC）不同结合模式构建类似脂肪组织结构的稳定型HIPPEs体系形成过程，分析其对乳化油滴大小调节作用；利用共聚焦-荧光标记和冷冻电镜等技术从界面解析乳化颗粒吸附行为并探究界面微观结构与流变、稳定性关系；借助溶剂干扰法等技术从体相分析BC自组装缠绕形成不同层面纤维网状结构的分子间作用力及对体系桥联稳定影响。全面揭示HIPPEs形成过程和对油滴聚结稳定调控机制，并评价其作为模拟脂肪组织的加工适宜性。研究结果为精准调控类似脂肪组织的HIPPEs体系构建提供理论依据，也为HIPPEs作为脂肪组织理想替代物的稳定设计提供新思路。

Fat mimetics are ideal replacers for animal fats in food processing. Currently, the formation and stabilization mechanism of high internal phase Pickering emulsion (HIPPEs) systems by simulating adipose tissue is not clear. Based on the results of previous studies that the high internal phase emulsion gels were fabricated by soybean protein particles (SPP), the concept of "assembly construction-stabilization analysis-processing evaluation" is proposed in this project. The formation process of a stable HIPPEs system similar to adipose tissue structure by different modes of SPP and bacterial cellulose (BC) are determined and the regulation effect of emulsifiers on the size of oil droplets are analyzed. The adsorption behavior of emulsifiers will be illuminated by confocal-fluorescent labeling and Cryo-SEM techniques. The relationship between emulsion microstructures and rheological, stabilization properties are investigated. By means of solvent-perturbation method and other techniques, the intermolecular forces of BC self-assembled network structure are analyzed and their effects on the bridging stability of system is studied. The purpose of study is to reveal the formation of HIPPEs and the regulation mechanism of oil droplet coalescence stability from the interface and volume phase. Subsequently, its macroscopic processing suitability of HIPPEs will be evaluated. Overall, the present project could provide a theoretical basis for further understanding of the precise and effective the fabrication of HIPPEs system similar to adipose tissue, as well as provide a new idea for the design of HIPPEs as ideal adipose tissue replacers.