拟南芥PRL1与RPA2A协同调控植物衰老进程的分子机制

衰老是植物生长发育的重要阶段。衰老过程伴随着基因组范围的转录重编程，大分子物质的降解和营养物质从衰老组织向新生组织和储藏器官的转移，对植物世代交替和作物产量形成具有重要意义。但是，目前对植物衰老调控机制的理解还很有限。本项目前期工作中发现，多效调控蛋白PRL1与复制蛋白RPA2A抑制黑暗碳饥饿胁迫诱导的衰老进程，PRL1与RPA2A存在直接互作，且两者均参与DNA损伤修复。在此基础上，本项目拟通过分析PRL1与RPA2A参与DNA损伤调控衰老的细胞与分子表征、明确PRL1与RPA2A参与DNA损伤修复调控衰老过程的遗传关系与解析PRL1-RPA2A互作调控黑暗碳饥饿胁迫诱导植物衰老的分子机制等三方面的工作，揭示PRL1与RPA2A参与调控植物衰老的生物学新功能，阐明DNA损伤修复与黑暗碳饥饿胁迫诱导植物衰老途径的相互调控关系，为人工改良作物衰老性状，提升作物产量与品质提供理论支撑。

Senescence is an important stage of the plant lifecycle. Senescence is accompanied by the genome-wide transcriptional reprogramming, degradation of macromolecules and the translocation of nutrients from senescent tissues to growing tissues and the storage organs, which are of great significance for plant generation alteration and crop yield formation. However, current understanding of the mechanism of plant senescence is still very limited. In our preliminary study, we have discovered that PRL1 and RPA2A inhibited the senescence process induced by dark and carbon deprivation. Moreover, we found that PRL1 interacted with RPA2A, and both of them were involved in DNA damage repair. Based on those findings, we proposed to explore the new biological functions of PRL1 and RPA2A and mechanism of PRL1 and RPA2A synergistically regulate plant senescence through the following three parts of works. First, analyze the cellular and molecule characterizations of PRL1 and RPA2A in DNA damage and regulation of senescence, second, elucidate the genetic relationship between PRL1 and RPA2A in the process of senescence induced by dark and carbon deprivation, and third, uncover the molecular mechanism of PRL1-RPA2A interaction element in regulating plant senescence. This work will reveal the new biological functions of PRL1 and RPA2A involved in regulating plant senescence, clarify the relationship between DNA damage repair and dark carbon starvation stress-induced plant senescence pathways, and provide theoretical support for artificially improving crop senescence traits and improving crop yield and quality.