胶胞炭疽菌效应蛋白CgCP1调控橡胶树防卫反应的分子机理研究

病原真菌分泌的效应蛋白进入植物细胞后，通过抑制寄主植物的防卫反应和调控植物的生理机能实现致病作用，是重要的致病因子。胶孢炭疽病菌引起的橡胶树炭疽病是橡胶减产的主要因素之一。前期课题组筛选到一个效应蛋白编码基因，命名为CgCP1。初步研究发现，CgCP1在胶孢炭疽菌对橡胶树的致病性中发挥重要作用，不仅能够引起叶片组织坏死和活性氧积累,还与橡胶树的NBS-LRR、几丁质酶和PRs相互作用。本研究拟在此基础上通过系统分析CgCP1 对橡胶树防卫反应的影响、分离鉴定CgCP1在橡胶树中的作用靶标，全面解析胶胞炭疽菌与植物互作的分子机制，这对建立新型橡胶树炭疽病防控策略和挖掘有效抗病基因资源具有重要的科学意义。本研究从效应蛋白与寄主植物免疫识别和互作的分子机制研究入手，通过分离鉴定效应蛋白CgCP1在橡胶树中的互作蛋白并研究其抗病功能，直接发现新的具有应用潜能的抗病关键组分，研究思路具有创新性。

As an important pathogenic factor of phytopathogenic fungi, the effectors are secreted into plant cells and play pathogenic roles by inhibiting and regulating the defense responses and physiological function of host plants. Rubber tree anthracnose is mainly caused by Colletotrichum gloeosporioides and lead to rubber production loss. An effector encoding gene called CgCP1 was cloned previously. Preliminary studies showed that CgCP1 play an important role in the pathogenicity of C. gloeosporioides on rubber tree. It was also found that CgCP1 could not only interact with NBS-LRR, chitinase and the pathogenesis-related proteins of rubber trees, but also cause accumulation of reactive oxygen species in mesophyll cell of rubber tree and tissue necrosis on tobacco leaves. In this project we propose to comprehensively analyze the molecular mechanism of the interaction between C. gloeosporioides and plants by systematically investigating the influence of CgCP1 on rubber tree defense system, isolating the target factors of CgCP1 in rubber trees, and identifying the function of these interacting proteins. The outcomes of this project will be helpful to build up new strategy for disease control of rubber tree and find effective disease resistance gene resources. The innovation of this project is that the effector will be used as a focal point to study the recognition mechanism between host plants and pathogens and to find new key genes for rubber tree disease resistance.