热敏灸肺俞穴调控OX40/OX40L影响下游PI3K/AKT信号通路对变应性鼻炎大鼠作用机制研究

据全球统计，变应性鼻炎发病率高达10%～25%。临床证据研究显示变应性鼻炎是热敏灸优势病种之一，但其效应机制尚不明确。变应性鼻炎发病机制与PI3K/AKT信号通路密切相关。前期研究发现，热敏灸可以延长变应性鼻炎大鼠潜伏期，改善鼻部症状，降低Th2介导表达的炎性因子，尤其是影响TSLP/OX40L表达。因此，本项目以变应性鼻炎大鼠为研究对象，以红外成像为热敏穴检测技术，以悬灸“肺俞”穴为干预措施，以OX40/OX40L共刺激信号分子及其下游PI3K/AKT信号通路为切入点，运用ELISA 、Real time PCR、Western blot、免疫荧光技术等多种技术手段，从OX40、OX40L、T-bet、GATA-3、t-Akt、p-Akt等与PI3K/AKT信号通路途径关键因子及启动调控环节中探索热敏灸减轻变应性鼻炎炎症反应的作用机制，为解释热敏灸干预变应性鼻炎提供实验依据。

According to global statistics, the number of allergic rhinitis patients has exceeded 500 million. Clinical evidence shows that allergic rhinitis is one of the dominant diseases of heat-sensitive moxibustion. The pathogenesis of allergic rhinitis is closely related to the PI3K/AKT signaling pathway. Previous studies have found that thermally sensitive moxibustion can prolong the incubation period of allergic rhinitis rats, improve nasal symptoms, and reduce th2-mediated inflammatory factors, especially affecting the expression of TSLP/OX40L. Therefore, this project took allergic rhinitis rats as the research object, took infrared imaging as the thermal point detection technology, took hanging moxibustion "lung shu" point as the intervention measure, took OX40/OX40L co-stimulating signal molecules and its downstream PI3K/AKT signaling pathway as the entry point, and used Real time-pcr, Western blot, immunofluorescence and other technical means. To explore the mechanism of heat-sensitive moxibustion in reducing allergic rhinitis from the aspects of OX40, OX40L, t-bet, gata-3, t-akt, p-akt and other key factors in the pathway of PI3K/ Akt signaling and the initiation and regulation, so as to provide a certain immunological mechanism to explain the clinical effectiveness of heat-sensitive moxibustion in the intervention of allergic rhinitis.