利用关联作图法解析马蹄莲MeJ-primed抗病蛋白编码基因内SNPs的遗传效应

免疫诱抗剂因能激活植物Priming响应并促进其抗病能力而被誉为“植物疫苗”，是目前植物保护研究的热点。但研究证明不同植物响应诱抗剂的能力是不同的，如MeJ处理可激活白色马蹄莲的Priming响应进而抵抗软腐病菌，但在彩色马蹄莲中却被抑制。因此如何选择和培育具有较强priming能力的基因型成为当前关注的重点。本项目结合蛋白组学和数量遗传学，在已鉴定106个马蹄莲属MeJ-primed抗病蛋白编码基因的基础上，以117份彩色马蹄莲为对象，首先通过基因克隆比对方法鉴定出候选关键SNP标记，并分析它们的遗传变异情况；而后利用关联作图方法解析它们对MeJ处理接菌表型的加性、显性与上位性效应；最后筛选出相关的主效遗传因子与功能标记位点。开展该工作不仅可以为探索植物Priming分子遗传基础问题提供参考，而且还可为今后开展马蹄莲及球根花卉的分子(基因)标记辅助抗病育种提供技术支撑。

Immune inducers are known as "plant vaccines", which is a hot spot in plant protection research, as they can activate the plant's priming defense response and confers disease resistance. However, numerous studies have proved that different plants have different abilities to respond to the inducers. For example, the compound MeJ treatment can activate the priming response in Zantedeschia aethiopica and thus resist soft rot bacteria, but it is inhibited in Z. hybrid. Therefore, the focus of current attention is to select and cultivate genotypes with strong MeJ-primed ability. This project intends to combine proteomics and quantitative genetics methods to resolve this problem. Based on the 106 MeJ-primed disease-resistant protein coding genes that have been identified by comparing the proteomics between Z. aethiopica and of Z. hybrid, we will first identify the key candidate SNP markers and detect its genetic variation in 117 Z. hybrid populations by gene cloning and sequences comparison etc. Then these SNPs will be associated with plant phenotype against Pectobacterium carotovorum to analyze its additive, dominant, and epistatic effects. Finally, the major genetic factors and functional marker loci associated with resistance phenotypic will be screened according to SNPs networks. This work will not only provide a very favorable reference for exploring scientific issues such as the molecular genetic basis of plant priming, but also provides theoretical and technical guidance for the development of genetic marker-assisted breeding in calla lily and other perennial bulbous flowers.