含ZIF-67@OGP涂层改性的钛植入体调控细胞交互对感染性骨修复的作用及机制研究

细菌感染及引起的严重炎症反应破坏了移植材料介导的生物材料-细胞以及细胞-细胞间的交互功能，导致感染性骨修复效果不佳。通过表面改性技术赋予钛植入体良好的抗菌、抗炎性能，加快感染性骨整合修复进程是提高钛植入体骨整合的重要手段。前期研究中，我们发现ZIF材料改性的钛基材具有良好的抗菌能力，同时利于成骨细胞粘附、增殖和分化，有效地提高感染性骨修复效果。鉴于此，本项目拟采用电化学沉积技术，在TNT表面构建含ZIF-67@OGP涂层结构，研究该涂层在体外条件下的抗菌、抗炎及对巨噬细胞和间充质干细胞的交互作用及分子机制。之后通过构建大鼠感染性骨缺损移植模型，进一步研究该涂层在植入初期抗菌、抗炎以及促骨再生的修复能力，重点探究ZIF-67@OGP涂层对炎性反应和促成骨化交互作用的调控规律和内在机制。本研究可为后续感染性骨修复植入体的设计和研发提供科学依据和理论基础。

Bacterial infections and the resulting severe inflammation response affect the material-cell, cell-cell interaction and lead to the undesirable infectious bone regeneration effect. Surface modifications of titanium implant to improve antibacterial, anti-inflammatory properties and facilitate its infected osseointegration is a vital method in the felid of metallic biomaterials modification research. In previous study, we revealed that ZIF modified titanium-based implant presents strong antibacterial ability, shows good cytocompatibility to osteoblast adhesion, proliferation and differentiation, and effectively improves the effect of infected bone regeneration. In view of this, OGP-loaded zeolitic imidazolate frameworks-67 (ZIF-67) is fabricated on titanium dioxide nanotubes (TNT) to prepare a TNT-ZIF-67@OGP coating structure via electrophoresis deposition（EPD). Promoted effects of coating structure on antibacterial，osteogenic differentiation of mesenchymal stem cells and anti-inflammation of RAW264.7 cells, as well as infected bone regeneration and repair in bone defect transplantation model are investigated. Importantly, the regulation effects of ZIF-67@OGP coating modified titanium substrates on the cell-cell interaction between inflammation and osteogenesis, and the underlying molecular mechanism will be further studied. This project could provide theoretical and practical indications for the design and development of antibacterial titanium implants for bone regeneration.