Basigin（BSG）是一种广泛表达多功能的蛋白，在多种疾病的组织/细胞表达上调，也是潜在的治疗靶点。近期研究表明，红细胞携带OK血型抗原的BSG膜蛋白是恶性疟原虫网织红细胞结合蛋白同源体5（PfRh5）的受体，PfRh5-BSG相互作用是恶性疟原虫入侵红细胞的关键。申请者在前期对OK/BSG多态性与PfRh5-BSG相互作用相关性的研究中发现miR-15b-5p、miR-99b-5p和miR-501-3p可能是OK/BSG的候选调控因子。我们拟通过细胞转染、双荧光素酶报告基因检测、qRT-PCR等实验方法分析miR-15b-5p、miR-99b-5p和miR-501-3p是否参与了BSG转录后调控及其靶点，和在OK/BSG表达中的作用。实验结果不仅有助于BSG相关的疟疾防治和其他生理病理过程的研究，也可能为以BSG为靶点的相关疾病治疗提供新的候选负调控因子，具有潜在的临床应用价值。

Basigin (BSG) is a multifunctional protein and expressed widely. BSG is up-regulated in tissues/cells in a variety of diseases, and is also the potential target for therapy. Recent studies indicate that BSG expressed on red blood cells, which carries OK blood group antigens, is the receptor of Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5). The PfRh5–BSG interaction is essential for erythrocyte invasion by Plasmodium falciparum. The applicant found that miR-15b-5p, miR-99b-5p, and miR-501-3p might be candidate regulation factors of OK/BSG in the previous research on the relativity between the polymorphisms of OK/BSG and the PfRh5–BSG interaction. We aims to analyze whether miR-15b-5p, miR-99b-5p, and miR-501-3p are involved in the post-transcriptional regulation of BSG, and their potential target sites and functions in the expression of OK/BSG by cell transfection, dual-luciferase reporter gene assay, qRT-PCR and other experimental methods. The expected results might be helpful for the study of malaria treatment and prevention as well as other physiological and pathological processes which are related to BSG, and also might provide new candidate negative regulators for the therapy of associated diseases targeting BSG. Therefore there might be potential clinical application value.