Ca2+缓解番茄低夜温光合障碍的SlCAS-PLC/IP3介导机制

我国冬春茬设施番茄生产中，常出现低夜温光合障碍，严重影响产量和品质。申请者前期研究发现，Ca2+具有缓解设施番茄低夜温光合障碍的作用，并发现SlCAS参与了Ca2+的这种调控作用。逆境激活的CAS可积累IP3，而PLC是合成IP3的关键酶。但SlCAS-PLC/IP3如何参与调控尚不明确。本项目拟以SlCAS和SlPLC基因敲除及超表达植株为试材，利用“Ca2+加低夜温(6℃) ”处理幼苗，验证SlCAS和SlPLC对低夜温和Ca2+的响应；通过分析光合荧光、叶绿体结构等，明确SlCAS介导Ca2+在缓解番茄低夜温光合障碍中的作用；分析SlCAS影响PLC酶活与IP3含量，挖掘受SlCAS调控的SlPLCs基因并验证其功能。通过上述研究，阐明Ca2+缓解番茄低夜温光合障碍的SlCAS-PLC/IP3介导机制，为利用外源Ca2+调控番茄低温光合障碍、提高冬春茬设施番茄产量和品质奠定基础。

Energy-saving facilities tomato production in China's winter and spring, often appear Photosynthetic obstacle caused by low night temperature, which seriously affect the yield and quality of tomato.The applicant has discovered that Ca2+ can alleviate Photosynthetic obstacle caused by low night temperature,and it has also been found that SlCAS are involved in this regulatory role of Ca2+. Exogenous Ca2+ induced CAS and further generate IP3, PLC is a key enzyme in the synthesis of IP3 and plays an important role in the response to Ca2+ and low temperature, But it is not clear how SlCAS-PLC/IP3 is involved in regulation.We obtain transgenic plants of SlCAS and SlPLC through the CRISPR/Cas9 knock out and overexpression techniques which are subjected to 6℃ LNT treatment after Ca2+ pretreatment. first,we realize the response to low night temperature and Ca2+ of SlCAS and SlPLC. We analysis the photosynthetic fluorescence parameters, guard cells, chloroplast structurea etc in a bid to clear the effect in alleviating photosynthetic disturbance of Ca2+ mediated in tomato by SlCAS in tomato in response to LNT. The influence of SlCAS on PLC enzyme activity and IP3 content was analyzed, and we furtherly excavate the SlPLCs gene regulated by SlCAS and verify its functionality, which will clear the effect in alleviating photosynthetic disturbance of Ca2+ mediated in tomato by SlPLC in tomato in response to LNT.Through the above research,we clear ultimately the alleviating photosynthetic disturbance mechanism of Ca2+mediated in tomato by SlCAS in response to LNT .Which will lay the foundation of theory and application for the regulation photosynthetic disturbance of Ca2+ in response to LNT,and improve the yield and quality of energy-saving facilities tomato in China's winter and spring.