基于超高分辨细胞微结构三维成像的磨玻璃肺结节瘤周放射组学特征研究

临床中磨玻璃结节肺腺癌浸润前病变与浸润性病变的诊疗方式及预后大不相同，亟待术前能够精准判别，传统影像学检查存在一定的局限性以及人为判断的主观性，目前尚缺乏客观精准判断二者的方法。申请人前期利用放射组学特征对磨玻璃结节肺腺癌浸润性进行鉴别等原创性研究，但该模型需要进一步优化且其具体病理细胞微结构基础有待进一步探索。研究团队利用超高分辨荧光显微成像技术打破了光学的衍射极限，可以对亚百纳米的细胞结构进行3D成像、重建及可视化，为本研究提供新的机遇。鉴于此，本研究提出假设:磨玻璃结节肺腺癌瘤周组织细胞微结构存在差异，放射组学可挖掘深层图像信息特征反映该差异。本研究拟通过磨玻璃结节瘤周组织放射组学特征构建并进一步优化肺腺癌浸润性的预测模型，从而为临床诊疗提供辅助工具，并通过超高分辨三维成像技术明确磨玻璃结节肺腺癌瘤周组织细胞微结构差异，也可能为今后研究肺腺癌发生发展和浸润提供新的思路。

Clinical diagnosis and treatment of lung adenocarcinoma appearing as ground-glassnodules are quite different due to the different prognosis of patients. Because of the limitations of traditional imaging examinations and the subjectivity of human judgment, there is still a lack of accurate and objective method of diagnose. Our previous research found that radiomic features extracted from peritumoral parenchyma can be used to differentiate the invasiveness of adenocarcinoma appearing as ground-glass nodules. But the model needs further optimization and the basis on microstructure of cells needs further exploration. The research team break the limit of optical diffraction by the super-resolution microscopy and can perform 3D imaging, reconstruction and visualization of sub-hundred nanometer cell structures，which providing new opportunities for the study. Therefore, a hypothesis is put forward in the study as follows: There may exist differences in the microstructure of cells around the lung adenocarcinoma in ground-glass nodules, and radiomics can extract deep information of images to reflect the difference. This study intends to construct and optimize the prediction model of lung adenocarcinoma based on the radiomics features of ground glass nodules, and provide an auxiliary tool for clinical diagnosis and treatment. Meantime, it may clarify the difference in the microstructure around the lung adenocarcinoma of ground-glass nodules by the super-resolution microscopy, which may provide new ideas for future research on the development and invasion of lung adenocarcinoma.